Integrin beta 7 Antibody (473207) [Fluorescein]

Human peripheral blood lymphocytes were stained with Mouse Anti-Human Integrin beta 7 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4669F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 473207
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Fluorescein

Integrin beta 7 Antibody (473207) [Fluorescein] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 7 long isoform
  Glu20-His723
  Accession # P26010
• Specificity: Detects human Integrin beta 7 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Integrin beta 1, beta 2, beta 3, beta 4, beta 5, beta 6, beta 8, recombinant mouse Integrin beta 4, or beta 7 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGB7
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin beta 7 Antibody (473207) [Fluorescein]

• Gut homing receptor beta subunit
• integrin beta 7 subunit
• Integrin beta 7
• integrin beta-7
• integrin, beta 7
• ITGB7

Background

Human Integrin beta 7 (also Integrin beta P and M290 IEL Ag) is a 120 kDa member of the Integrin beta chain family of molecules. It is a type I transmembrane glycoprotein that contains a 704 amino acid (aa) extracellular domain (ECD) (aa 20‑723) and a 52 aa cytoplasmic tail. The ECD contains one von Willebrand factor A domain (aa 150‑389) and four cysteine‑rich EGF‑like repeats. One splice variant exists that shows a deletion of aa 501‑648. Integrin beta 7 forms a nondisulfide linked heterodimer with alpha 4 and alpha E integrins. alpha 4 beta 7 binds fibronectin, VCAM-1 and MADCAM1, while alpha E beta 7 binds E-cadherin. The ECD of human beta 7 is 87% aa identical to the ECD of mouse beta 7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 7 Antibody (FAB4669F)(1)

Publication using FAB4669F

• Sereti I, Estes J, Thompson W, Morcock D, Fischl M, Croughs T, Beq S, Lafaye de Micheaux S, Yao M, Ober A, Wilson E, Natarajan V, Imamichi H, Boulassel M, Lederman M, Routy J Decreases in colonic and systemic inflammation in chronic HIV infection after IL-7 administration. PLoS Pathog, 2014-01-30;10(1):e1003890. 2014-01-30 [PMID: 24497828] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: ITGB7
• Uniprot:
  • P26010()