Integrin beta 6 Antibody [Unconjugated]

• Reactivity: Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin beta 6 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 6
  Gly22-Asn706
  Accession # Q9Z0T9
• Specificity: Detects mouse Integrin beta 6 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ITGB6
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin beta 6 Antibody [Unconjugated]

• Integrin beta 6
• integrin beta-6
• integrin, beta 6
• ITGB6

Background

Integrin beta 6 is a type I transmembrane Integrin beta subunit that heterodimerizes with alpha V subunits in the epithelial restricted integrin alpha V/ beta 6. The extracellular domain of mouse Integrin beta 6 contains a collagen binding von-Willebrand factor A domain and multiple truncated EGF-like motifs. Mouse Integrin beta 6 extracellular domain shares 96% and 91% amino acid sequence identity with the corresponding regions of the rat and human protein, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 6 Antibody (AF2389)(5)

Publications using AF2389

• JA Pace, R Bronstein, Y Guo, Y Yang, CC Estrada, N Gujarati, DJ Salant, J Haley, AB Bialkowska, VW Yang, JC He, SK Mallipattu Podocyte-specific KLF4 is required to maintain parietal epithelial cell quiescence in the kidney Science Advances, 2021;7(36):eabg6600. 2021 [PMID: 34516901] (IHC, Mouse)
• T Xu, Z Lu, Z Xiao, F Liu, Y Chen, Z Wang, S Zhu, Y Song Myofibroblast induces hepatocyte-to-ductal metaplasia via laminin-?v&beta6 integrin in liver fibrosis Cell Death Dis, 2020;11(3):199. 2020 [PMID: 32251270] (ICC, Mouse)
• J Bi, L Koivisto, A Pang, M Li, G Jiang, S Aurora, Z Wang, GR Owen, J Dai, Y Shen, D Grenier, M Haapasalo, L Häkkinen, H Larjava Suppression of ?v?6 Integrin Expression by Polymicrobial Oral Biofilms in Gingival Epithelial Cells Sci Rep, 2017;7(1):4411. 2017 [PMID: 28667248] (Western Blot, Human)
• Mohazab L, Koivisto L, Jiang G, Kytomaki L, Haapasalo M, Owen G, Wiebe C, Xie Y, Heikinheimo K, Yoshida T, Smith C, Heino J, Hakkinen L, McKee M, Larjava H Critical role for alphavbeta6 integrin in enamel biomineralization. J Cell Sci, 2013;126(0):732-44. 2013 [PMID: 23264742] (Western Blot, Mouse)
• Xu K, Nieuwenhuis E, Cohen BL, Wang W, Canty AJ, Danska JS, Coultas L, Rossant J, Wu MY, Piscione TD, Nagy A, Gossler A, Hicks GG, Hui CC, Henkelman RM, Yu LX, Sled JG, Gridley T, Egan SE Lunatic Fringe-mediated Notch signaling is required for lung alveogenesis. Am. J. Physiol. Lung Cell Mol. Physiol., 2010;298(1):L45-56. 2010 [PMID: 19897741] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: ITGB6
• Uniprot:
  • Q9Z0T9()