Integrin beta 6 Antibody [Unconjugated]

The effect of beta 6 integrin-deficiency on inflammatory cytokine expression. (A) SiRNA knockdown of beta 6 integrin in the GECs after a 48-h transfection relative to beta -actin detected by Western blotting. SiRNA that is not homologous to any human gene (C) was used as a control. (B) The effect of beta 6 integrin knockdown on IL1B expression in biofilm-treated (60 µg protein/ml; 32 h) and non-treated GECs determined by RT-qPCR. Mean ± SEM is presented, n = 3. (C) The effect of TGF-beta 1 signaling inhibitor SB431542 (5 µM) on ITGB6 and IL1B expression in biofilm-treated (60 µg protein/ml; 32 h) and non-treated GECs. Mean ± SEM, n = 3. (D) GECs were treated with TGF-beta 1 (2 ng/ml), heated biofilm extract (60 µg protein/ml) or a combination of both for 32 h and analyzed for ITGB6 expression by RT-qPCR. Mean ± SEM, n = 3. (E) GECs were treated with TGF-beta 1 (2 ng/ml) for 0–120 min and analyzed for Smad2 phosphorylation (activation) relative to total Smad2 by Westerm blotting. (F and G) GECs were treated with TGF-beta 1 (2 ng/ml), heated biofilm extract (60 µg protein/ml) or a combination of both for 30 min and analyzed for Smad2 phosphorylation relative to total Smad2 by Westerm blotting. The ratio of phosphorylated Smad2 to total Smad2 was determined from triplicated experiments. *p < 0.05; **p < 0.01; ***p < 0.001. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28667248), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin beta 6 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 6
  Gly22-Asn706
  Accession # Q9Z0T9
• Specificity: Detects mouse Integrin beta 6 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ITGB6
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin beta 6 Antibody [Unconjugated]

• Integrin beta 6
• integrin beta-6
• integrin, beta 6
• ITGB6

Background

Integrin beta 6 is a type I transmembrane Integrin beta subunit that heterodimerizes with alpha V subunits in the epithelial restricted integrin alpha V/ beta 6. The extracellular domain of mouse Integrin beta 6 contains a collagen binding von-Willebrand factor A domain and multiple truncated EGF-like motifs. Mouse Integrin beta 6 extracellular domain shares 96% and 91% amino acid sequence identity with the corresponding regions of the rat and human protein, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 6 Antibody (AF2389)(9)

Publications using AF2389

• F Quaglia, SR Krishn, K Sossey-Ala, PS Rana, E Pluskota, PH Park, CD Shields, S Lin, P McCue, AV Kossenkov, Y Wang, DW Goodrich, SY Ku, H Beltran, WK Kelly, E Corey, M Klose, C Bandtlow, Q Liu, DC Altieri, EF Plow, LR Languino The NOGO receptor NgR2, a novel alphaVbeta3 integrin effector, induces neuroendocrine differentiation in prostate cancer Scientific Reports, 2022-11-07;12(1):18879. 2022-11-07 [PMID: 36344556] (Western Blot, Human)
• Shiv Ram Krishn, Vaughn Garcia, Nicole M. Naranjo, Fabio Quaglia, Christopher D. Shields, Maisha A. Harris et al. Small extracellular vesicle-mediated ITGB6 siRNA delivery downregulates the alpha V beta 6 integrin and inhibits adhesion and migration of recipient prostate cancer cells Cancer Biology & Therapy 2022-12-31 [PMID: 35188070]
• Nicole M. Naranjo, Israa Salem, Maisha A. Harris, Lucia R. Languino IFIT3 (interferon induced protein with tetratricopeptide repeats 3) modulates STAT1 expression in small extracellular vesicles Biochemical Journal 2021-11-12 [PMID: 34622927]
• JA Pace, R Bronstein, Y Guo, Y Yang, CC Estrada, N Gujarati, DJ Salant, J Haley, AB Bialkowska, VW Yang, JC He, SK Mallipattu Podocyte-specific KLF4 is required to maintain parietal epithelial cell quiescence in the kidney Science Advances, 2021-09-03;7(36):eabg6600. 2021-09-03 [PMID: 34516901] (IHC, Mouse)
• T Xu, Z Lu, Z Xiao, F Liu, Y Chen, Z Wang, S Zhu, Y Song Myofibroblast induces hepatocyte-to-ductal metaplasia via laminin-?v&amp;beta6 integrin in liver fibrosis Cell Death Dis, 2020-03-23;11(3):199. 2020-03-23 [PMID: 32251270] (ICC, Mouse)
• J Bi, L Koivisto, A Pang, M Li, G Jiang, S Aurora, Z Wang, GR Owen, J Dai, Y Shen, D Grenier, M Haapasalo, L Häkkinen, H Larjava Suppression of ?v?6 Integrin Expression by Polymicrobial Oral Biofilms in Gingival Epithelial Cells Sci Rep, 2017-06-30;7(1):4411. 2017-06-30 [PMID: 28667248] (Western Blot, Human)
• Melissa Ducceschi, Lisa G. Clifton, Stephen A. Stimpson, Andrew N. Billin Post-transcriptional regulation of ITGB6 protein levels in damaged skeletal muscle Journal of Molecular Histology 2014-06-01 [PMID: 24488487] (Immunohistochemistry, Human, Mouse)
• Mohazab L, Koivisto L, Jiang G, Kytomaki L, Haapasalo M, Owen G, Wiebe C, Xie Y, Heikinheimo K, Yoshida T, Smith C, Heino J, Hakkinen L, McKee M, Larjava H Critical role for alphavbeta6 integrin in enamel biomineralization. J Cell Sci, 2012-12-21;126(0):732-44. 2012-12-21 [PMID: 23264742] (Western Blot, Mouse)
• Xu K, Nieuwenhuis E, Cohen BL, Wang W, Canty AJ, Danska JS, Coultas L, Rossant J, Wu MY, Piscione TD, Nagy A, Gossler A, Hicks GG, Hui CC, Henkelman RM, Yu LX, Sled JG, Gridley T, Egan SE Lunatic Fringe-mediated Notch signaling is required for lung alveogenesis. Am. J. Physiol. Lung Cell Mol. Physiol., 2009-11-06;298(1):L45-56. 2009-11-06 [PMID: 19897741] (IHC-P, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: ITGB6
• Uniprot:
  • Q9Z0T9()