Integrin beta 6 Antibody (437211) [Unconjugated]

HT‑29 human colon adenocarcinoma cell line was stained with Human Integrin beta 6 Monoclonal Antibody (Catalog # MAB4155, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B).

Integrin beta 6 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Mouse Anti-Human Integrin beta 6 Monoclonal Antibody (Catalog # MAB4155) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Integrin beta 6 was detected in immersion fixed HT‑29 human colon adenocarcinoma cell line (positive) and A549 human lung carcinoma cell line (negative) cells using Mouse Anti-Human Integrin beta 6 Monoclonal Antibody (Catalog # MAB4155) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready, ICC/IF
• Clone: 437211
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin beta 6 Antibody (437211) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Integrin alpha V beta 6
  Phe31-Val992 (Integrin alpha V) and Gly22-Asn707 (Integrin beta 6)
  Accession # P18564
• Specificity: Detects human Integrin beta 6 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGB6
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin beta 6 Antibody (437211) [Unconjugated]

• Integrin beta 6
• integrin beta-6
• integrin, beta 6
• ITGB6

Background

Integrin beta 6 (ITG beta 6) is a 95 kDa member of the Integrin beta family. It forms noncovalent heterodimers with Integrin alpha V and appears on epithelia following injury or inflammation. It activates TGF-beta and assists keratinocyte migration. Human Integrin beta 6 is a type I transmembrane glycoprotein that is 767 aa in length. It contains a 688 aa extracellular domain (ECD) (aa 22‑709) that incorporates a 241 aa VWF-A domain. Over aa 22‑707, human integrin beta 6 ECD shares 90% and 93% aa sequence identity with mouse and pig integrin beta 6 ECD, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 6 Antibody (MAB4155)(5)

Publications using MAB4155

• Rouaud, L;Baudin, L;Gautier-Isola, M;Van Meerbeeck, P;Feyereisen, E;Blacher, S;van Baren, N;Kridelka, F;Lucas, S;Noel, A; Spatial Distribution of Non-Immune Cells Expressing Glycoprotein A Repetitions Predominant in Human and Murine Metastatic Lymph Nodes Cancers 2023-11-28 [PMID: 38067325] (Flow Cytometry, Human)
• I Malenica, J Adam, S Corgnac, L Mezquita, E Auclin, I Damei, L Grynszpan, G Gros, V de Montpré, D Planchard, N Théret, B Besse, F Mami-Choua Integrin-alphaV-mediated activation of TGF-beta regulates anti-tumour CD8 T cell immunity and response to PD-1 blockade Nature Communications, 2021-09-01;12(1):5209. 2021-09-01 [PMID: 34471106] (Flow Cytometry, Human)
• Melody G. Campbell, Anthony Cormier, Saburo Ito, Robert I. Seed, Andrew J. Bondesson, Jianlong Lou et al. Cryo-EM Reveals Integrin-Mediated TGF-beta Activation without Release from Latent TGF-beta Cell 2/1/2020 [PMID: 31955848]
• Ring A, Nguyen C, Smbatyan G et al. CBP/beta-Catenin/FOXM1 Is a Novel Therapeutic Target in Triple Negative Breast Cancer Cancers (Basel). 12/19/2018 [PMID: 30572639]
  
  Details:
  Citation used the Unpurified format of this antibody.
• Cabezon R, Sintes J, Llinas L, Benitez-Ribas D Analysis of HLDA9 mAbs on plasmacytoid dendritic cells. Immunol. Lett., 2010-10-07;134(2):167-73. 2010-10-07 [PMID: 20933012] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: ITGB6
• Uniprot:
  • P18564()