Integrin alpha 5 beta 1 Antibody (BMC5)

Immunohistochemistry: Integrin alpha 5 beta 1 Antibody (BMC5) [NBP2-29788] - Rat anti-Mouse Integrin alpha5beta1 staining of mouse gut.

• Reactivity: Mu
• Applications: Flow, Func, IHC, IP, IHC-P (-)
• Clone: BMC5
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Integrin alpha 5 beta 1 Antibody (BMC5) Summary

• Description: Relevant Entrez Gene Numbers: NM_002205.2
• Immunogen: Mouse embryonal carcinoma P19 cells with myeloma P3X63Ag9.653 cells.
• Specificity: Antibody was raised against mouse integrin VLA-5 (alpha5beta1), a fibronectin receptor. The antibody was prepared by fusion of spleen cells from a Fisher rat immunized with mouse embryonal carcinoma P19 cells with myeloma P3X63Ag9.653 cells. Cell lines tested to express alpha5beta1, include: P19 cells, mouse melanoma B16F1 cells, mouse squamous carcoma SCC cells and thymocytes.
• Protein/Peptide Type: Affinity Matrices
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: ITGA5
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10ul/ml
  • Functional 10ul/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 2 uL
• Application Notes: Inhibition of cell adhesion to fibronectin: 10 ul/mL Note that where cell adhesion to fibronectin involving other fibronectin receptors, such as VLA-4 (alpha4beta1) integrin, is involved, inhibition of cell adhesion by BMC5 alone may not be complete. Immunohistochemistry on acetone-fixed frozen sections only; formalin fixation is not recommended.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: No Preservative
• Purity: Unpurified

Notes

Relevant Entrez Gene Numbers: NM_002205.2

Alternate Names for Integrin alpha 5 beta 1 Antibody (BMC5)

• CD49 antigen-like family member E
• CD49e
• Fibronectin receptor subunit alpha
• fibronectin receptor, alpha polypeptide
• fibronectin receptor, alpha subunit
• FNRA
• Integrin alpha 5 beta 1
• integrin alpha-5
• Integrin alpha-F
• integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• ITGA5/SLC9B1 fusion
• very late activation protein 5, alpha subunit
• VLA-5
• VLA5A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 5 beta 1 Antibody (NBP2-29788)(3)

Publications using NBP2-29788

• Lima E Silva R, Mirando AC, Tzeng SY et al. Anti-angiogenic collagen IV-derived peptide target engagement with ?(v)?(3) and ?(5)?(1) in ocular neovascularization models iScience 2023-02-17 [PMID: 36844452] (Immunohistochemistry-Frozen, Mouse)
• Tang Y, Luo K, Tan J et al. Laminin alpha 4 promotes bone regeneration by facilitating cell adhesion and vascularization Acta biomaterialia 2021-03-09 [PMID: 33711525] (WB)
• Smolders SM, Swinnen N, Kessels S et al. Age-specific function of alpha5B1 integrin in microglial migration during early colonization of the developing mouse cortex Glia. 2017-04-18 [PMID: 28417486] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: ITGA5
• Uniprot:
  • P08648()