Insulysin/IDE Antibody (JJ0949)

Western Blot: Insulysin/IDE Antibody (JJ0949) [NBP2-66907] - Analysis of Insulin degrading enzyme on hybrid fish (crucian-carp) heart tissue lysate using anti-Insulin degrading enzyme antibody at 1/500 dilution.

Immunohistochemistry-Paraffin: Insulysin/IDE Antibody (JJ0949) [NBP2-66907] - Analysis of mouse colon tissue using anti-Insulin degrading enzyme antibody. Counter stained with hematoxylin.

Western Blot: Insulysin/IDE Antibody (JJ0949) [NBP2-66907] - Analysis of Insulin degrading enzyme on different lysates using anti-Insulin degrading enzyme antibody at 1/1,000 dilution. Positive control: Lane 1: zebrafish Lane 2: Hela Lane 3: K562

Immunohistochemistry-Paraffin: Insulysin/IDE Antibody (JJ0949) [NBP2-66907] - Analysis of human colon cancer tissue using anti-Insulin degrading enzyme antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Insulysin/IDE Antibody (JJ0949) [NBP2-66907] - Analysis of paraffin-embedded human gastric carcinoma tissue using anti-Insulin degrading enzyme antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Insulysin/IDE Antibody (JJ0949) [NBP2-66907] - Analysis of human liver cancer tissue using anti-Insulin degrading enzyme antibody. Counter stained with hematoxylin.

Product Discontinued

Insulysin/IDE Antibody (JJ0949) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Insulysin/IDE aa 1-106 / 1019. (SwissProt: P14735 Human; SwissProt: Q9JHR7 Mouse; SwissProt: P35559 Rat)
• Localization: Cytoplasm, Cell membrane, Secreted.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IDE
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Insulysin/IDE Antibody (JJ0949)

• Abeta-degrading protease
• EC 3.4.24
• EC 3.4.24.56
• FLJ35968
• IDE
• INSDEGM
• Insulin protease
• Insulinase
• insulin-degrading enzyme
• Insulysin

Background

Insulysin was identified nearly a century ago as an enzyme responsible for the degradation of insulin in cells, although the precise interactions between insulin and insulysin remain elusive. Human insulysin was cloned in 1988, and shown to be a 118 kDa protein that exists primarily as a homodimer, and perhaps also complexed with other molecules. The sequence is well conserved between humans, rats and mice, and the antibody recognizes these species. Insulysin is a metalloproteinase of the clan ME, family M16, which contains an active site HxxEH, a reversal of the canonical HExxH zinc binding motif. Considered a zinc metalloproteinase, the activity of insulysin can be blocked with EDTA or 1-10 phenanthroline. In addition to the active metalloproteinase domain, insulysin contains a second metalloproteinase site which is considered catalytically inactive, and is thought to assist in substrate binding. Insulysin is most closely related to the bacterial proteinase pitrilysin, (the human orthologue of which appears to be MPRP1) and the mammalian proteinsae nardilysin. Generally thought to be a cytoplasmic protein, insulysin has been isolated from many different tissues and cell lines, and can degrade intact insulin, insulin B chain, glucagon, denatured hemoglobin, alpha amyloid protein, TGF alpha and amylin. Recent work implicates insulysin in clearing beta amyloid plaques from the brain, and has generated much interest in Alzheimer's disease research. The pH optimum for insulysin is basic, pH 8.5, which also distinguishes it from other metalloproteinases. Insulin degrading enzyme (IDE) has a preferential affinity for insulin such that the presence of insulin will inhibit IDE mediated degradation of other substrates. IDE degrades a variety of other peptides including atrial natriuretic peptide and amylin. IDE catabolizes A beta and has been implicated as a candidate enzyme responsible for the degradation and clearance of A beta in the brain. IDE has also been shown to degrade the APP intracellular domain (AICD), a product of gamma secretase cleaved APP that may function in nuclear signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Insulysin/IDE Antibody (NBP2-66907)(1)

Publication using NBP2-66907

• Klingler F, Naumann L, Schlossbauer P et al. A novel system for glycosylation engineering by natural and artificial miRNAs Metabolic engineering 2023-03-09 [PMID: 36906118] (WB, Hamster)
  
  Details:
  Dilution used in WB 1:100

Bioinformatics

• Gene Symbol: IDE