Insulysin/IDE Products

Antibodies
Insulysin/IDE Antibody
Insulysin/IDE Antibody
NBP2-38994
Species: Hu, Mouse, Rat
Applications: WB, ICC/IF, IHC, IHC-P
Host: Rabbit Polyclonal
Insulysin/IDE Antibody
Insulysin/IDE Antibody
NB100-68148
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
Host: Goat Polyclonal
Insulysin/IDE Antibody (33450 ...
Insulysin/IDE Antibody (334501) [U...
MAB2496
Species: Hu
Applications: WB, ICFlow
Host: Mouse Monoclonal
Lysates
Insulysin/IDE Overexpression ...
Insulysin/IDE Overexpression Lysat...
NBL1-11816
Applications: WB
Proteins
Insulysin/IDE Recombinant Pro ...
Insulysin/IDE Recombinant Protein
H00003416-P01
Species: Hu
Applications: WB, ELISA, PA
Insulysin/IDE Partial Recombi ...
Insulysin/IDE Partial Recombinant ...
H00003416-Q01
Species: Hu
Applications: WB, ELISA, PA
Insulysin/IDE Recombinant Pro ...
Insulysin/IDE Recombinant Protein ...
NBP2-38994PEP
Species: Hu
Applications: AC
RNAi
Insulysin/IDE RNAi
Insulysin/IDE RNAi
H00003416-R01
Species: Hu

Description

Insulysin was identified nearly a century ago as an enzyme responsible for the degradation of insulin in cells, although the precise interactions between insulin and insulysin remain elusive. Human insulysin was cloned in 1988, and shown to be a 118 kDa protein that exists primarily as a homodimer, and perhaps also complexed with other molecules. The sequence is well conserved between humans, rats and mice, and the antibody recognizes these species. Insulysin is a metalloproteinase of the clan ME, family M16, which contains an active site HxxEH, a reversal of the canonical HExxH zinc binding motif. Considered a zinc metalloproteinase, the activity of insulysin can be blocked with EDTA or 1-10 phenanthroline. In addition to the active metalloproteinase domain, insulysin contains a second metalloproteinase site which is considered catalytically inactive, and is thought to assist in substrate binding. Insulysin is most closely related to the bacterial proteinase pitrilysin, (the human orthologue of which appears to be MPRP1) and the mammalian proteinsae nardilysin. Generally thought to be a cytoplasmic protein, insulysin has been isolated from many different tissues and cell lines, and can degrade intact insulin, insulin B chain, glucagon, denatured hemoglobin, alpha amyloid protein, TGF alpha and amylin. Recent work implicates insulysin in clearing beta amyloid plaques from the brain, and has generated much interest in Alzheimer's disease research. The pH optimum for insulysin is basic, pH 8.5, which also distinguishes it from other metalloproteinases. Insulin degrading enzyme (IDE) has a preferential affinity for insulin such that the presence of insulin will inhibit IDE mediated degradation of other substrates. IDE degrades a variety of other peptides including atrial natriuretic peptide and amylin. IDE catabolizes A beta and has been implicated as a candidate enzyme responsible for the degradation and clearance of A beta in the brain. IDE has also been shown to degrade the APP intracellular domain (AICD), a product of gamma secretase cleaved APP that may function in nuclear signaling.

Bioinformatics

Entrez Human
Mouse
Rat
Uniprot Human
Human
Human
Human
Mouse
Product By Gene ID 3416
Alternate Names
  • EC 3.4.24
  • FLJ35968
  • Insulysin
  • EC 3.4.24.56
  • Insulinase
  • Abeta-degrading protease
  • insulinase
  • Insulin protease
  • INSULYSIN
  • insulin-degrading enzyme

Research Areas for Insulysin/IDE

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Lipid and Metabolism

Bioinformatics Tool for Insulysin/IDE

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