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Rat Insulin Receptor beta ELISA Kit (Chemiluminescence)

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Rat Insulin Receptor beta ELISA Kit (Chemiluminescence) - Standard Curve Reference
Rat Insulin Receptor beta ELISA Kit (Chemiluminescence) - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids.
Standard Curve Range
0.16-10 ng/mL
Sensitivity
0.09 ng/mL

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Rat Insulin Receptor beta ELISA Kit (Chemiluminescence) Summary

Specificity
This kit recognizes Rat INSR-Beta in samples. No significant cross-reactivity or interference between Rat INSR-Beta and analogues was observed.
Standard Curve Range
0.16-10 ng/mL
Sensitivity
0.09 ng/mL
Assay Type
Sandwich-CLIA
Inter-Assay
CV% < 9.46%
Intra-Assay
CV% < 10.09%
Spike Recovery
91-114%
Sample Volume
100 uL
Kit Type
ELISA Kit (Chemiluminescence)
Gene
INSR

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Certificate of Analysis
  3. Concentrated Biotinylated Detection Ab (100×)
  4. Concentrated HRP Conjugate (100×)
  5. Concentrated Wash Buffer (25×)
  6. HRP Conjugate Diluent
  7. Micro CLIA Plate(Dismountable)
  8. Plate Sealer
  9. Product Description
  10. Reference Standard & Sample Diluent
  11. Reference Standard
  12. Substrate Reagent A
  13. Substrate Reagent B

Alternate Names for Rat Insulin Receptor beta ELISA Kit (Chemiluminescence)

  • CD220 antigen
  • CD220
  • EC 2.7.10
  • EC 2.7.10.1
  • HHF5
  • insulin receptor
  • IR

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Insulin was detected in immersion fixed beta TC‑6 mouse beta cell insulinoma cell line using Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 10&amp;nbsp;µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog&amp;nbsp;# NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Species: Bv, Hu, Mu
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IRS1 Antibody
(OTI3G10)
NBP2-45435
Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
IGF-I/IGF-1
[Unconjugated]
291-G1
1 μg/lane of Recombinant Human IGF-I/IGF-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at&amp;nbsp;7&amp;nbsp;kDa.
Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line. The ED50 for this effect is 0.3‑1.5&amp;nbsp;ng/mL.
MALDI-TOF analysis of Recombinant Human IGF-I/IGF-1.The major peak corresponds to the calculated molecular mass, 7650 Da. The minor peak at 7873 is a matrix-associated artifact of the MALDI-TOF.
(8 Reviews)
(172 Publications)
Species: Hu
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AKT [p Ser473]
Antibody - Pan Specif
AF887
Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100&amp;nbsp;ng/mL Human&amp;nbsp;PDGF&amp;nbsp;(Catalog&amp;nbsp;# 120‑HD) for 15&amp;nbsp;minutes and MCF‑7 human breast cancer cell line untreated or treated with 100&amp;nbsp;ng/mL&amp;nbsp;Recombinant Human IGF-1 (Catalog&amp;nbsp;# 291‑G1) for 15&amp;nbsp;minutes. PVDF membrane was probed with 0.5&amp;nbsp;µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog&amp;nbsp;# AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog&amp;nbsp;# HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group&amp;nbsp;1.
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog&amp;nbsp;# 291-G1) for 15&amp;nbsp;minutes, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for Phospho-Akt (S473) at approximately 60&amp;nbsp;kDa (as indicated) using 5&amp;nbsp;µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog&amp;nbsp;# AF887). This experiment was conducted&amp;nbsp;under&amp;nbsp;reducing&amp;nbsp;conditions and using the 12-230 kDa separation system.
Serum-starved MCF‑7 human breast cancer cells were unstimulated (light orange filled histogram) or activated with&amp;nbsp;100&amp;nbsp;ng/mL Recombinant human IGF-1 (Catalog&amp;nbsp;# 291-G1, dark orange filled histogram) for 15&amp;nbsp;minutes, then stained with Rabbit Anti-Human/Mouse/Rat Phospho‑Akt (S473) Antigen Affinity‑purified Polyclonal Antibody (Catalog&amp;nbsp;# AF887) or control antibody (Catalog&amp;nbsp;# AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog&amp;nbsp;# F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
(26 Publications)
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
Glut4 Antibody -
BSA Free
NBP1-49533
Flow (Intracellular): Glut4 Antibody [NBP1-49533] - An intracellular stain was performed on HepG2 with NBP1-49533 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.
Western Blot: Glut4 Antibody [NBP1-49533] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-Glut4 0.5 ug/ml in non-fat milk. Undiffer: undifferentiated; Differ: Differentiated. Image from verified customer review.
Western Blot: Glut4 Antibody [NBP1-49533] - Glycolysis-related genes and proteins were upregulated in differentiated podocytes. Representative blot images of glycolysis-related proteins (n =3). d All proteins were normalized to tubulin and compared to UDPs. *P &lt; 0.05, **P &lt; 0.01, determined by t test. Data are shown as the means +/- SD. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41419-020-2481-5), licensed under a CC-BY licence.
(25 Publications)
Species: Hu, Mu, Pl, Rt
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-P, KD, WB
IRS2 Antibody
[Unconjugated]
AF6347
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF‑7 human breast cancer cell line. PVDF Membrane was probed with 2&amp;nbsp;µg/mL of Sheep Anti-Human IRS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6347) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog&amp;nbsp;# HAF016). A specific band was detected for IRS2 at approximately 170-190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
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IGF-II/IGF2
[Unconjugated]
292-G2
1 μg/lane of Recombinant Human IGF&amp;#8209;II/IGF2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7&amp;nbsp;kDa.
Recombinant Human IGF-II/IGF2 (Catalog&amp;nbsp;# 292‑G2) stimulates cell proliferation in the MCF-7 human breast cancer cell line. The ED50 for this effect is 1.5‑6&amp;nbsp;ng/mL.
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Species: Hu
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IGF-I R/IGF1R
Antibody (33255) [Unc ...
MAB391
Western blot shows lysates of NTera‑2 human testicular embryonic carcinoma cell line, SK‑Mel‑28 human malignant melanoma cell line, and G361 human melanoma cell line. PVDF membrane was probed with 1&amp;nbsp;µg/mL of Mouse Anti-Human/Mouse IGF-I R/IGF1R Monoclonal Antibody (Catalog # MAB391) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog&amp;nbsp;# HAF007). A specific band was detected for IGF-I R/IGF1R at approximately 275&amp;nbsp;kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 2.
IGF-I R/IGF1R was detected in perfusion fixed paraffin-embedded sections of mouse heart using Mouse Anti-Human/Mouse IGF-I&amp;nbsp;R/IGF1R Monoclonal Antibody (Catalog # MAB391) at 15&amp;nbsp;µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte&amp;#8482; HRP Polymer Antibody (Catalog&amp;nbsp;# VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
MCF&amp;#8209;7 human breast cancer cell line was stained with Mouse Anti-Human IGF&amp;#8209;I&amp;nbsp;R/IGF1R Monoclonal Antibody (Catalog&amp;nbsp;# MAB391, filled histogram) or isotype control antibody (Catalog&amp;nbsp;# MAB002, open histogram) followed by anti-mouse IgG PE-conjugated secondary antibody (Catalog&amp;nbsp;# F0102B). View our protocol for Staining Membrane-associated Proteins.&amp;nbsp;&amp;nbsp;
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PYK2/FAK2 Antibody
(SC06-15)
NBP2-67627
Immunocytochemistry/Immunofluorescence: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Staining PYK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.
Immunocytochemistry/Immunofluorescence: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Staining PYK2 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X-100/PBS.
Immunocytochemistry/Immunofluorescence: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Staining PYK2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X-100/PBS.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
ERK1 Antibody (1E5)
NBP2-22203
Flow Cytometry: ERK1 Antibody (1E5) [NBP2-22203] - Flow cytometric analysis of Hela cells using ERK1 mouse mAb (blue) and negative control (red).
Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of paraffin-embedded bladder cancer tissues using ERK1 mouse mAb with DAB staining.
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
EGF [Unconjugated]
236-EG
1 µg/lane of Recombinant Human EGF was resolved&amp;nbsp;with SDS-PAGE&amp;nbsp; and visualized&amp;nbsp;by silver staining under reducing (R) conditions, showing a single band at 6 kDa.
Recombinant Human EGF (Catalog # 236‑EG) stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line. The ED50 for this effect is 20‑100 pg/mL.
(26 Reviews)
(581 Publications)
Species: Hu
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TNF-alpha
[Unconjugated]
210-TA/CF
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human TNF-alpha (Catalog # 210‑TA/CF) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
(702 Publications)
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EGFR Antibody
[Unconjugated]
AF231
EGFR was detected in immersion fixed&amp;nbsp;frozen sections of human&amp;nbsp;skin using Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) at 1&amp;nbsp;µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte&amp;#8482; HRP Polymer Antibody (Catalog&amp;nbsp;# VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
EGFR was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) at 1&amp;nbsp;µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog&amp;nbsp;# NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA‑MB‑231 human breast cancer cell line. PVDF membrane was probed with 1&amp;nbsp;µg/mL of Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog&amp;nbsp;# AF231) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF017). A specific band was detected for EGFR at approximately 175&amp;nbsp;kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(1 Review)
(13 Publications)
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, WB
ERK2 Antibody
(OTI6E5)
NBP1-47842
Immunohistochemistry-Paraffin: ERK2 Antibody (6E5) [NBP1-47842] - Endometrium tissue within the normal limits using antiMAPK1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,Dilution 1:50)
Immunoprecipitation: ERK2 Antibody (6E5) [NBP1-47842] - TrueMab monoclonal anti-ERK2 antibody (Negative control: IP without adding anti-ERK2 antibody). For each experiment, 500ul of DDK tagged ERK2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of antibody.
Immunohistochemistry-Paraffin: ERK2 Antibody (6E5) [NBP1-47842] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ERK2 mouse monoclonal antibody.
Species: Ca, Hu, Pm, Mu, Rt
Applications: IHC, IHC-P, IP, WB
Isoleucyl tRNA
synthetase Antibody
H00003376-B01P
Western Blot: Isoleucyl tRNA synthetase Antibody [H00003376-B01P] - Analysis of IARS expression in HepG2.
Western Blot: Isoleucyl tRNA synthetase Antibody [H00003376-B01P] - Analysis of IARS expression in transfected 293T cell line by IARS polyclonal antibody. Lane1:IARS transfected lysate(116.05 KDa). Lane2:Non-transfected lysate.
Western Blot: Isoleucyl tRNA synthetase Antibody [H00003376-B01P] - Analysis of IARS expression in human placenta.
Species: Hu
Applications: WB
PI 3-Kinase p110
beta/PIK3CB Antibody
NBP1-33116
Immunocytochemistry/Immunofluorescence: PI 3-Kinase p110 beta/PIK3CB Antibody [NBP1-33116] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Western Blot: PI 3-Kinase p110 beta/PIK3CB Antibody [NBP1-33116] - Sample (50 ug of whole cell lysate) A: C2C12 B: C2C12+AEA10mM antibody diluted at 1:1000.
Western Blot: PI 3-Kinase p110 beta/PIK3CB Antibody [NBP1-33116] - Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE; antibody diluted at 1:1000.
(2 Publications)
Species: Bv, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
PI 3-Kinase p110
gamma Antibody
NBP2-15071
Western Blot: PI 3-Kinase p110 gamma Antibody [NBP2-15071] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 gamma antibody [C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: PI 3-Kinase p110 gamma Antibody [NBP2-15071] - Human Breast Cancer, using PI3K gamma antibody. Antigen Retrieval: Trilogy&amp;#8482; (EDTA based, pH 8.0) buffer, 15min
Western Blot: PI 3-Kinase p110 gamma Antibody [NBP2-15071] - Whole cell extract (30ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 gamma antibody [C3], C-term diluted at 1:1000.
(3 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
PIK3CA Antibody
NBP2-19804
Western Blot: PIK3CA Antibody [NBP2-19804] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 alpha antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunohistochemistry-Paraffin: PIK3CA Antibody [NBP2-19804] - Human Breast Cancer, using PI3 kinase p110 alpha antibody. Antigen Retrieval: Trilogy&amp;#8482; (EDTA based, pH 8.0) buffer, 15min.
Western Blot: PIK3CA Antibody [NBP2-19804] - PIK3CA Antibody Sample (50 ug of whole cell lysate) A: C2C12 B: C2C12+AEA10mM NBP2-19804 diluted at 1:1000.
(3 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
PI 3-Kinase p110
delta Antibody (OTI1D ...
NBP2-46404
Immunohistochemistry: PI 3-Kinase p110 delta Antibody (1D11) [NBP2-46404] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PI 3-Kinase p110 delta Antibody (1D11) [NBP2-46404] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PI 3-Kinase p110 delta Antibody (1D11) [NBP2-46404] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P
PI 3-Kinase p85
alpha Antibody (A3-D0
NBP2-67058
Flow Cytometry: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Analysis of MCF-7 cells with PI3-kinase p85 subunit alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondar
Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Staining of Hela cells using anti-PI3-kinase p85 subunit alpha Mouse mAb.
Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Staining of SH-SY-5Y cells using anti-PI3-kinase p85 subunit alpha Mouse mAb.
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB

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  Rat Insulin Receptor beta ELISA Kit (Chemiluminescence) Manual
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Bioinformatics

Gene Symbol INSR