Insulin R/CD220 Antibody (243524) [Alexa Fluor® 594]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
243524
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 594
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Insulin R/CD220 Antibody (243524) [Alexa Fluor® 594] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Insulin R/CD220
His28-Lys944
Accession # NP_001073285
Specificity
Detects human Insulin R/CD220 in direct ELISAs.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
INSR
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Insulin R/CD220 Antibody (243524) [Alexa Fluor® 594]

  • CD 220
  • CD220 antigen
  • CD220
  • EC 2.7.10
  • EC 2.7.10.1
  • HHF5
  • INSR
  • Insulin R
  • insulin receptor
  • InsulinR
  • IR

Background

The Insulin Receptor (INS R) and insulin-like growth factor-1 receptor (IGF-I R) constitute a subfamily of receptor tyrosine kinases (1-4). The two receptors share structural similarity as well as overlapping intracellular signaling events, and are believed to have evolved through gene duplication from a common ancestral gene. INS R cDNA encodes a type I transmembrane single chain preproprotein with a putative 27 amino acid residues (aa) signal peptide. The large INS R extracellular domain is organized into two successive homologous globular domains, which are separated by a Cysteine-rich domain, followed by three fibronectin type III domains. The intracellular region contains the kinase domain sandwiched between the juxtamembrane domain used for docking insulin-receptor substrates (IRS), and the carboxy-terminal tail that contains two phosphotyrosine-binding sites. After synthesis, the single chain INS R precursor is glycosylated, dimerized and transported to the Golgi apparatus where it is processed at a furin-cleavage site within the middle fibronectin type III domain to generate the mature disulfide-linked  alpha 2 beta 2 tetrameric receptor. The alpha subunit is localized extracellularly and mediates ligand binding while the transmembrane beta subunit contains the cytoplasmic kinase domain and mediates intracellular signaling. As a result of alternative splicing, two INS R isoforms (A and B) that differ by the absence or presence, respectively, of a 12 aa residue sequence in the carboxyl terminus of the alpha subunit exist. Whereas the A isoform is predominantly expressed in fetal tissues and cancer cells, the B isoform is primarily expressed in adult differentiated cells. Both the A and B isoforms bind insulin with high-affinity, but the A isoform has considerably higher affinity for IGF‑I and IGF-II. Ligand binding induces a conformational change of the receptor, resulting in ATP binding, autophosphorylation, and subsequent downstream signaling. INS R signaling is important in metabolic regulation, but may also contribute to cell growth, differentiation and apoptosis. Mutations in the INS R gene have been linked to insulin-resistant diabetes mellitus, noninsulin-dependent diabetes mellitus and leprechaunism, an extremely rare disorder characterized by abnormal resistance to insulin that results in a variety of distinguishing characteristics, including growth delays and abnormalities affecting the endocrine system. INS R is highly conserved between species, rat INS R shares 94% and 97% aa sequence homology with the human and mouse receptor, respectively.

  1. Nakae, J. et al. (2001) Endoc. Rev. 22:818.
  2. De Meyts, P. and J. Whittaker (2002) Nature Rev. Drug Disc. 1:769.
  3. Kim, J.J. and D. Accili (2002) Growth Hormone and IGF Res. 12:84.
  4. Sciacca, L. et al. (2003) Endocrinology 144:2650.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol INSR
Uniprot