INSIG-2 Overexpression Lysate

Images

 
Western Blot: INSIG-2 Overexpression Lysate (Adult Normal) [NBL1-12004] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for INSIG-2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

INSIG-2 Overexpression Lysate Summary

Description
Transient overexpression lysate of insulin induced gene 2 (INSIG2) The lysate was created in HEK293T cells, using Plasmid ID RC205274 and based on accession number NM_016133. The protein contains a C-MYC/DDK Tag.
Gene
INSIG2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
24.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for INSIG-2 Overexpression Lysate

  • INSIG2 membrane protein
  • INSIG-2
  • insulin induced gene 2
  • insulin induced protein 2
  • insulin-induced gene 2 protein
  • MGC26273

Background

The protein encoded by this gene is highly similar to the protein product encoded by gene INSIG1. Both INSIG1 protein and this protein are endoplasmic reticulum proteins that block the processing of sterol regulatory element binding proteins (SREBPs) by binding to SREBP cleavage-activating protein (SCAP), and thus prevent SCAP from escorting SREBPs to the Golgi. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for INSIG-2 Lysate (NBL1-12004) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for INSIG-2 Lysate (NBL1-12004) (0)

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Other Available Formats

NBL1-12004

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SREBP1 (sterol-regulatory-element-binding protein 2) is a basic-helix-loop-helix-leucine zipper (bHLH-ZIP) transcription factor. It regulates sterol and cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g....  Read full blog post.

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Bioinformatics

Gene Symbol INSIG2
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