Recombinant Human Inorganic Pyrophosphatase/PPA1 Protein

Product Discontinued

Recombinant Human Inorganic Pyrophosphatase/PPA1 Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-289 of Human Inorganic Pyrophosphatase/PPA1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSGFST EERAAPFSLE YRVFLKNEKG QYISPFHDIP IYADKDVFHM VVEVPRWSNA KMEIATKDPL NPIKQDVKKG KLRYVANLFP YKGYIWNYGA IPQTWEDPGH NDKHTGCCGD NDPIDVCEIG SKVCARGEII GVKVLGILAM IDEGETDWKV IAINVDDPDA ANYNDINDVK RLKPGYLEAT VDWFRRYKVP DGKPENEFAF NAEFKDKDFA IDIIKSTHDH WKALVTKKTN GKGISCMNTT LSESPFKCDP DAARAIVDAL PPPCESACTV PTDVDKWFHH QKN

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PPA1
• Purity: >95% pure by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 35.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 2 mM DTT, 20% glycerol, 200 mM NaCl
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >95% pure by SDS-PAGE

Alternate Names for Recombinant Human Inorganic Pyrophosphatase/PPA1 Protein

• diphosphate phosphohydrolase
• EC 3.6.1.1
• inorganic diphosphatase
• inorganic pyrophosphatase 1
• inorganic pyrophosphatase
• IOPPP
• IOPPPMGC111556
• IPP1
• PP1
• PPA1
• PPase
• PPcytosolic inorganic pyrophosphatase
• pyrophosphatase (inorganic) 1
• pyrophosphatase (inorganic)
• pyrophosphatase 1
• Pyrophosphate phospho-hydrolase
• SID6-8061

Background

PPA1 (Pyrophosphatase) belongs to the PPase family. This protein is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. The hydrolysis of inorganic pyrophosphate (PPi) to two phosphate ions is utilized in many biochemical pathways to render reactions effectively irreversible. Inorganic pyrophosphatase catalyzes this hydrolysis reaction in the early steps of lipid degradation, a prominent example of this phenomenon. By promoting the rapid hydrolysis of pyrophosphate (PPi), Inorganic pyrophosphatase provides the driving force for the activation of fatty acids destined for oxidation. Recombinant human PPA1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PPA1