Inorganic Pyrophosphatase/PPA1 Antibody (840941) [Alexa Fluor® 647]

• Applications: WB
• Clone: 840941
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

Inorganic Pyrophosphatase/PPA1 Antibody (840941) [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human Inorganic Pyrophosphatase/PPA1
  Met1-Asn289
  Accession # Q15181
• Specificity: Detects human Inorganic Pyrophosphatase/PPA1 in direct ELISAs and human, mouse, and rat Inorganic Pyrophosphatase/PPA1 in Western blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

PPA1 (Inorganic pyrophosphatase 1; also PPase and IOPPP) is a 32-36 kDa cytoplasmic member of the PPase family of enzymes. It is ubiquitously expressed, and acts on di- (or pyro) phosphate, generating orthophosphate in a Mg⁺-dependent manner. This activity can both generate energy for cells, or in the case of osteoblasts, provide raw material for calcification. The consumption of pyrophosphate may also remove inhibitors of enzymes such as guanylyl cyclase, and PPA1 itself is also reported to stimulate gene expression. Human PPA1 is 289 amino acids (aa) in length. There is one pyrophosphatase domain between aa 42-255, and two utilized acetylation sites at Lys 57 and Lys228. PPA1 is known to form homodimers. There is one potential alternative start site at Met46. Full-length human PPA1 shares 94% aa sequence identity with mouse PPA1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.