Importin alpha 3/KPNA4 Antibody

Images

 
Western Blot: Importin alpha 3/KPNA4 Antibody [NB100-1374] - Analysis of KPNA4 / Importin alpha 3 in Human Testis lysate using this antibody at 0.5 ug/ ml. Primary incubated for 1 hour. Detected by western blot using ...read more
Immunohistochemistry-Paraffin: Importin alpha 3/KPNA4 Antibody [NB100-1374] - Staining of paraffin embedded Human Small Intestine at 5 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: Importin alpha 3/KPNA4 Antibody [NB100-1374] - Paraffin embedded Human Testis at 3ug/ml. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Importin alpha 3/KPNA4 Antibody Summary

Immunogen
Peptide with sequence C-NSSANVPTEGFQF corresponding to C-Terminus according to NP_002259.1.
Localization
Cytoplasmic and Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
KPNA4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 - 1 ug/ml
  • Immunohistochemistry 1 - 2 ug/ml
  • Immunohistochemistry-Paraffin 1 - 2 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 55-60 kDa band observed in human testis and ovary lysates (predicted MW of approx. 58 kDa band according to NP_002259.1). IHC-P: Human testis show strong nuclear staining of some cells in the seminiferous tubules. Human small intestine shows pixulate staining in nuclei of enterocytes.
Positive Control
Testis Lysate (NB820-59266)
Importin alpha 3/KPNA4 Lysate (NBL1-12372)
Publications
Read Publication using NB100-1374.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Importin alpha 3/KPNA4 Antibody

  • Importin alpha 3
  • Importin alpha Q1
  • importin subunit alpha-4
  • importin-alpha-Q1
  • IPOA3
  • karyopherin alpha 4 (importin alpha 3)
  • KPNA4
  • MGC12217
  • MGC26703
  • Qip1
  • QIP12
  • QIP1Karyopherin subunit alpha-4
  • SRP3

Background

The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Importin alpha 3/KPNA4 Antibody (NB100-1374)(1)

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol KPNA4
Entrez
Uniprot