Importin alpha 2/KPNA2 Antibody

Western Blot: Importin alpha 2/KPNA2 Antibody [NB100-1371] - Staining of Hela lysate (RIPA buffer, 35 ug total protein per lane). Antibody at 0.03 ug/mL. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody [NB100-1371] - Staining of paraffin embedded Human Testis. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Importin alpha 2/KPNA2 Antibody Summary

• Immunogen: Peptide with sequence C-QVQDGAPGTFNF corresponding to C-Terminus according to NP_002257.1.
• Localization: Cytoplasmic
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KPNA2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.03 - 0.1 ug/mL
• Application Notes: WB: Approx 55 kDa band observed in lysates of cell line HeLa (calculated MW of 57.9 kDa according to NP_002257.1).
• Control:

  Importin alpha 2/KPNA2 Overexpression Lysate
  HeLa Whole Cell Lysate

Reactivity Notes

Expected from sequence similarity: Mouse, Rat, Canine

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Importin alpha 2/KPNA2 Antibody

• Importin alpha 1
• Importin alpha 2
• importin-alpha-P1
• IPOA1
• karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
• Karyopherin subunit alpha-2
• KPNA2
• Pendulin
• QIP2
• QIP2importin alpha 2
• RAG cohort 1
• RAG cohort protein 1
• RCH1
• RCH1importin subunit alpha-2
• SRP1
• SRP1alpha
• SRP1-alpha

Background

The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Importin alpha 2/KPNA2 Antibody (NB100-1371)(2)

Publications using NB100-1371

• Park Jh, Ryu Sj, Kim Bj Et Al. Disruption of nucleocytoplasmic trafficking as a cellular senescence driver Experimental & molecular medicine 2021-06-01 [PMID: 34188179]
• Cuomo CA, Kirch SA, Gyuris J et al. Rch1, a protein that specifically interacts with the RAG-1 recombination-activating protein. Proc Natl Acad Sci U S A 1994-06-21 [PMID: 8016130]

Bioinformatics

• Gene Symbol: KPNA2
• Entrez:
  • 3838(Human)
  • 16647(Mouse)
  • 85245(Rat)
• Uniprot:
  • NP_002257.(Human)