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Importin 11 Antibody

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Western Blot: Importin 11 Antibody [NB100-56484] - Analysis of Importin-11 in HeLa lysate in the 1) absence and 2) presence of immunizing peptide using this antibody at 1 ug/ml.
Western Blot: Importin 11 Antibody [NB100-56484] - Analysis of Importin-11 in HeLa lysate in the 1) absence and 2) presence of immunizing peptide using this antibody at 1 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, PmSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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Catalog# & Formulation Size Price
Azide Free
NBP2-33209
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Importin 11 Antibody Summary

Immunogen
A portion of amino acids 1-50 of human Importin-11 was used as the immunogen (NP_001128251).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IPO11
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
112 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Importin 11 Lysate (NBL1-12014)
Publications
Read Publication using NB100-56484.

Reactivity Notes

Bovine, Chimpanzee, Dog, Human, Mouse, Rat, Rhesus Monkey. Predicted cross-reactivity based on sequence identity: Xenopus (94%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.2% gelatin
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Importin 11 Antibody

  • imp11
  • importin 11
  • Ran binding protein 11
  • Ran-binding protein 11
  • RANBP11
  • RanBP11importin-11
  • SLRN

Background

Importin-11 is a novel member of Karyopherin family. Karyopherins are transport receptors that mediate the transport of protein and RNA across the nucleus and cytoplasm. Importin-11 consists of an N-terminal Ran-binding domain, a central cargo-binding region and C-terminal region. It interacts with Ran-GTPase and constitutively shuttles between the nucleus and cytoplasm. Reports suggest Importin-11 primarily mediates the transport of ribosomal protein L12 and UbcM2, an E2 ubiquitin-conjugating enzyme.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: UBE2E3 Antibody (7E8) [NBP2-03819] - Staining of paraffin-embedded Human prostate tissue using anti-UBE2E3 mouse monoclonal antibody.
Flow Cytometry: UBE2E3 Antibody (7E8) [NBP2-03819] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-UBE2E3 antibody, and then analyzed by flow cytometry.
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Immunocytochemistry/Immunofluorescence: DBT Antibody [NBP1-89522] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining in human kidney and pancreas tissues using anti-DBT antibody. Corresponding DBT RNA-seq data are presented for the same tissues.
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Immunohistochemistry-Paraffin: Lipoamide Dehydrogenase Antibody (6D5) [NBP2-01109] - Staining of paraffin-embedded Human pancreas tissue using anti-Lipoamide Dehydrogenase mouse monoclonal antibody.
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Western Blot: ALDH9A1 Antibody [NBP1-89150] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
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Positive Control Lysate(s)

Importin 11 Overexpression Lysate (Native)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol IPO11
Entrez
Uniprot