ILT5/CD85a/LILRB3 Antibody (1057017) [Unconjugated]

Human neutrophils with CD15 costain cells were stained with Mouse Anti-Human/Mouse SSEA‑1 PE‑conjugated Monoclonal Antibody (Catalog # FAB2155P) and either (A) Mouse Anti-Human LILRB3/CD85a/ILT5 Monoclonal Antibody (Catalog # MAB18061) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

PBMC with CD14 costain were stained with either (A) Mouse Anti-Human LILRB3/CD85a/ILT5 Monoclonal Antibody (Catalog # MAB18061) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 1057017
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

ILT5/CD85a/LILRB3 Antibody (1057017) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell, HEK293-derived human LILRB3/CD85a/ILT5
  Gly24-Glu443
  Accession # O75022
• Specificity: Detects human LILRB3/CD85a/ILT5 in direct ELISA.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ILT5/CD85a/LILRB3 Antibody (1057017) [Unconjugated]

• CD85 antigen-like family member A
• CD85a antigen
• CD85a
• HL9Immunoglobulin-like transcript 5
• ILT5
• ILT-5
• ILT5CD85a
• Leukocyte immunoglobulin-like receptor 3
• leukocyte immunoglobulin-like receptor subfamily B member 3
• leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains)
• LILRB3
• LIR3
• LIR3CD85A
• LIR-3MGC138403
• member 3
• Monocyte inhibitory receptor HL9
• PIRB

Background

Leukocyte immunoglobulin-like receptor subfamily B (LILRB3), also known as ILT5, LIR3, and CD85a, is an immunoglobulin superfamily member that is involved in immune regulation. Subfamily B members have cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events via phosphatase SHP-1. Subfamily A members are activating receptors that lack ITIMs and signal through association with FcR gamma (1, 2). Mature LILRB3 is a highly polymorphic 85‑95 kDa glycoprotein that consists of a 420 amino acid (aa) extracellular domain (ECD) with four Ig-like domains, a 21 aa transmembrane segment, and a 167 aa cytoplasmic domain with three ITIMs (3). Alternative splicing generates an isoform with a 17 aa insertion in the juxtamembrane ECD. In mouse and rat, the LILRB3 gene encodes the PIR-B protein which has six Ig-like domains. Rodent PIR-B and human LILRB3 share 55% aa sequence identity within common regions of their ECDs. LILRB3 is expressed on the surface of peripheral monocytes, neutrophils, eosinophils, basophils, and mast cell progenitors (4-6). Triggering of LILRB3 inhibits the activation of macrophages, mast cells, neutrophils, basophils, and B cells (5, 7). On osteoclast precursors, LILRB3 ligation inhibits RANK L/TRANCE or M-CSF induced differentiation (8). LILRB3 can also bind to ligands exposed on necrotic tumor cells (9). Both PIR-B and LILRB3 are receptors for S. aureus, and activation of these receptors by bacteria influences the innate immune response triggered by TLRs (3). R&D Systems in-house testing indicates that LILRB3 binds to
Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families (10). In the mouse CNS, PIR-B functions as a receptor for the myelin proteins Nogo, MAG, and OMgp and mediates their inhibitory action on neurite outgrowth and axon regeneration (11). Upon binding to MAG, PIR-B associates with TrkB and NGF R/p75 in cerebellar granule neurons (12).

1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
2. Anderson, K.J. and R.L. Allen (2009) Immunology 127:8.
3. Nakayama, M. et al. (2007) J. Immunol. 178:4250.
4. Tedla, N. et al. (2003) Proc. Natl. Acad. Sci. USA 100:1174.
5. Sloane, D.E. et al. (2004) Blood 104:2832.
6. Tedla, N. et al. (2008) J. Leukoc. Biol. 83:334.
7. Uehara, T. et al. (2001) J. Clin. Invest. 108:1041.
8. Mori, Y. et al. (2008) J. Immunol. 181:4742.
9. Jones, D.C. et al. (2016) Oncotarget PMID 26769854.
10. Zheng, J. et al. (2012) Nature 485:656.
11. Atwal, J.K. et al. (2008) Science 322:967.
12. Fujita, Y. et al. (2011) Cell Death Dis. 2:e198.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O75022(Human)