PIR-B Antibody [Unconjugated]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

PIR-B Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse PIR-B
  Gly24-Gly635
  Accession # AAH26937
• Specificity: Detects mouse PIR-B in direct ELISAs and Western blots. In direct ELISAs, less than 40% cross-reactivity with recombinant mouse PIR-A is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Pirb
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PIR-B Antibody [Unconjugated]

• CD85a
• HL9
• ILT5
• ILT-5
• leukocyte immunoglobulin like receptor B3
• LILRA6
• LILRB3
• LIR3
• LIR-3
• PIRB
• PIR-B

Background

Mouse PIR-B is a 125 kDa type I transmembrane glycoprotein with six Ig-like domains in its extracellular domain (ECD) and four ITIM-like sequences in its cytoplasmic domain. The ECD of PIR-B is highly homologous to the ECDs of multiple mouse PIR-A receptors (92‑99% amino acid sequence homology), which have short cytoplasmic tails lacking ITIM motifs. PIR-A receptors have a charged residue in their transmembrane domain that facilitates interaction with ITAM-containing adaptor molecules. Whereas PIR-A receptors deliver activation signals, PIR-B can inhibit receptor-mediated activation signaling. PIR-A and PIR-B have been shown to bind various mouse MHC class I molecules. They have been proposed to be orthologs of human leukocyte immunoglobulin-like receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PIR-B Antibody (AF2754)(5)

Publications using AF2754

• Yuki Kawaguchi, Junpei Matsubayashi, Yutaka Kawakami, Ryohei Nishida, Yuji Kurihara, Kohtaro Takei LOTUS suppresses amyloid beta -induced dendritic spine elimination through the blockade of amyloid beta binding to PirB Molecular Medicine 12/12/2022 [PMID: 36510132]
• Xuying Li, Qian Zhai, Xingchun Gou, Minxue Quan, Yansong Li, Xiaohua Zhang et al. Involvement of Paired Immunoglobulin-Like Receptor B in Cognitive Dysfunction Through Hippocampal-Dependent Synaptic Plasticity Impairments in Mice Subjected to Chronic Sleep Restriction Molecular Neurobiology 3/1/2023 [PMID: 36417104]
• Kimura T, Endo S, Inui M, Saitoh S, Miyake K, Takai T Endoplasmic Protein Nogo-B (RTN4-B) Interacts with GRAMD4 and Regulates TLR9-Mediated Innate Immune Responses. J Immunol, 2015-04-27;194(11):5426-36. 2015-04-27 [PMID: 25917084] (Western Blot, Mouse)
• Y Fujita, R Takashima, S Endo, T Takai, T Yamashita The p75 receptor mediates axon growth inhibition through an association with PIR-B Cell Death & Disease 9/1/2011 [PMID: 21881600]
• Raiker SJ, Lee H, Baldwin KT Oligodendrocyte-myelin glycoprotein and Nogo negatively regulate activity-dependent synaptic plasticity. J. Neurosci., 2010-09-15;30(37):12432-45. 2010-09-15 [PMID: 20844138] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: Pirb
• Uniprot:
  • AAH26937(Mouse)