ILKAP/PP2C delta Antibody (499526)

Western blot shows lysates of PC-3 human prostate cancer cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat ILKAP/PP2Cd Monoclonal Antibody (Catalog # MAB4554) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ILKAP/PP2Cd at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 499526
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

ILKAP/PP2C delta Antibody (499526) Summary

• Immunogen: E. coli-derived recombinant human ILKAP
  Met1-His392
  Accession # Q9H0C8
• Specificity: Detects endogenous human, mouse and rat ILKAP in Western blots. In Western blots, this antibody does not cross-react with recombinant human PP2C alpha , PP2C beta or PPC2 gamma .
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ILKAP
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ILKAP/PP2C delta Antibody (499526)

• DKFZp434J2031
• EC 3.1.3.16
• FLJ10181
• ILKAP
• integrin-linked kinase-associated serine/threonine phosphatase 2C
• integrin-linked kinase-associated serine/threonine phosphatase
• MGC4846
• PP2C delta
• PP2C-DELTA
• protein phosphatase 2c, delta isozyme

Background

Integrin Linked Kinase 1 Associated Phosphatase (ILKAP), also called PP2Cδ, is a serine/threonine phosphatase that is stimulated by manganese but inhibited by magnesium ions and is insensitive to the phosphatase inhibitor okadaic acid. It binds to ILK1 and dephosphorylates it, decreasing the stimulation of the GSK-3 beta pathway by cell adhesion without affecting Akt signaling. Transfection studies have shown that ILKAP activity inhibits cell cycle progression from the G1 to S phase. ILKAP is expressed in many tissues but is highest in liver, and skeletal and cardiac muscle. Its gene is found in a region of chromosome 2 that is frequently deleted in oral cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ILKAP
• Uniprot:
  • Q9H0C8()