ILF2 Antibody (OTI6F1)

Western Blot: ILF2 Antibody (OTI6F1) [NBP1-47811] - Analysis of extracts (35ug) from 9 different cell lines by using anti-ILF2 monoclonal antibody.

Immunocytochemistry/Immunofluorescence: ILF2 Antibody (OTI6F1) [NBP1-47811] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ILF2.

Immunohistochemistry-Paraffin: ILF2 Antibody (OTI6F1) [NBP1-47811] - Staining of paraffin-embedded Kidney tissue using anti-ILF2 mouse monoclonal antibody.

Western Blot: ILF2 Antibody (OTI6F1) [NBP1-47811] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ILF2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ILF2.

• Reactivity: Hu, Mu, Ca
• Applications: WB, ICC/IF, IHC
• Clone: OTI6F1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

ILF2 Antibody (OTI6F1) Summary

• Description: Novus Biologicals Mouse ILF2 Antibody (OTI6F1) (NBP1-47811) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full-length protein expressed in 293T cell transfected with human ILF2 expression vector
• Specificity: This antibody is specific for Homo sapiens interleukin enhancer binding factor 2, 45kDa (ILF2).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ILF2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:500
• Theoretical MW: 43.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ILF2 Antibody (OTI6F1)

• interleukin enhancer binding factor 2, 45kD
• interleukin enhancer binding factor 2, 45kDa
• interleukin enhancer-binding factor 2
• MGC8391
• NF45PRO3063
• Nuclear factor of activated T-cells 45 kDa
• nuclear factor of activated T-cells, 45-kDa

Background

Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2.In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of thisgene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ILF2
• Entrez:
  • 3608(Human)