ILF1 Antibody

Western Blot: ILF1 Antibody [NB100-88148] - Western Blot: whole cell lysates from MDA-MB-231 and SUM159 cells were loaded with 50 ug/lane. 10% SDS-PAGE. ILF1 antibody (NB100-88148) was used for primary antibody: 1:2000, 4C, overnight. Image from verified customer review.

Immunohistochemistry-Paraffin: ILF1 Antibody [NB100-88148] - Human small cell lung cancer. Antibody used at a dilution of 1:200 (1ug/ml).

Western Blot: ILF1 Antibody [NB100-88148] - Detection of Human FoxK2 on HeLa whole cell lysate using NB100-88148. FoxK2 was also immunoprecipitated by rabbit anti-FoxK2 antibody NB100-88147.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

ILF1 Antibody Summary

• Description: Novus Biologicals Rabbit ILF1 Antibody (NB100-88148) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 635 and 660 of human forkhead box K2 using the numbering given in entry NP_004505.2 (GeneID 3607).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FOXK2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Immunoprecipitation 2 - 5 ug/mg lysate
  • Western Blot 1:100-1:2000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ILF1 Antibody

• Cellular transcription factor ILF-1
• forkhead box K2
• forkhead box protein K2
• FOXK1
• ILF-1
• ILF1ILF
• interleukin enhancer binding factor 1
• Interleukin enhancer-binding factor 1

Background

The protein encoded by this gene contains a fork head DNA binding domain. This protein can bind to the purine-rich motifs of the HIV long terminal repeat (LTR), and to the similar purine-rich motif in the interleukin 2 (IL2) promoter. It may be involved in the regulation of viral and cellular promoter elements. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for ILF1 Antibody (NB100-88148) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Yongkang Yang WB Human 10/22/2022

Summary

• Application: Western Blot
• Sample Tested: MDA-MB-231,SUM-159PT
• Species: Human
• Lot: A1

Bioinformatics

• Gene Symbol: FOXK2
• Entrez:
  • 3607(Human)
• Uniprot:
  • NP_004505.2(Human)