SF20/MYDGF Antibody [Unconjugated]

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SF20/MYDGF Antigen Affinity-purified Antibody (Catalog # AF1147) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for SF20/MYDGF at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of DU145 human prostate carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SF20/MYDGF at approximately 22 kDa (as indicated) using 25 µg/mL of Goat Anti-Human SF20/MYDGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1147) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated

SF20/MYDGF Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human SF20/MYDGF
  Val32-Leu173
  Accession # Q969H8
• Specificity: Detects human SF20/MYDGF in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse SF20/MYDGF is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MYDGF
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SF20/MYDGF Antibody [Unconjugated]

• C19orf10
• chromosome 19 open reading frame 10
• EUROIMAGE1875335
• IL25
• IL27
• IL27w
• interleukin 27 working designation
• interleukin-25
• Ly6elg
• MYDGF
• myeloid-derived growth factor
• R33729_1
• SF20
• Stromal cell-derived growth factor SF20
• UPF0556 protein C19orf10

Background

Human SF20 (Stromal cell-derived growth factor SF20, C19orf10, MYDGF1, Myeloid-derived growth factor1 also formerly known as IL25) is a 15 kDa (predicted)  bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and/or repair after myocardial infarction. SF20 stimulates endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1 mediated signaling pathway. It is thought to inhibit cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway and is involved in endothelial cell proliferation and angiogenesis. Human SF20 shares 87% aa identity with mouse SF20.              

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SF20/MYDGF Antibody (AF1147)(1)

Publication using AF1147

• V Bortnov, DS Annis, FJ Fogerty, KT Barretto, KB Turton, DF Mosher Myeloid-derived growth factor is a resident endoplasmic reticulum protein J. Biol. Chem., 2018-06-28;0(0):. 2018-06-28 [PMID: 29954947] (ICC, Western Blot, Human)

Bioinformatics

• Gene Symbol: MYDGF
• Uniprot:
  • Q969H8()