IL-5R alpha/CD125 Antibody [Alexa Fluor™ Plus 488]

• Reactivity: Mu
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor Plus 488

IL-5R alpha/CD125 Antibody [Alexa Fluor™ Plus 488] Summary

• Specificity: Detects mouse IL‑5 R alpha /CD125 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human (rh) IL‑5 R alpha is observed and less than 1% cross‑reactivity with recombinant mouse (rm) IL-4 R, rhIL-9 R, rhIL-13 R alpha 1, and rhIL-13 R alpha 2 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-5R alpha/CD125 Antibody [Alexa Fluor™ Plus 488]

• CD125 antigen
• CD125
• CDw125CD125
• HSIL5R3
• IL-5 R alpha
• IL-5 receptor subunit alpha
• IL5R alpha
• IL-5R subunit alpha
• IL5R
• IL5RA
• IL-5Ra
• IL-5R-alpha
• interleukin 5 receptor type 3
• interleukin 5 receptor, alpha
• interleukin-5 receptor alpha chain
• interleukin-5 receptor alpha subunit
• interleukin-5 receptor subunit alpha
• MGC26560

Background

Interleukin‑5 Receptor alpha (IL‑5 R alpha ), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology (1‑3). Mature mouse IL‑5 R alpha consists of a 322 amino acid (aa) extracellular domain (ECD) with a WSxWS motif and a four cysteine motif, a 22 aa transmembrane segment, and a 54 aa cytoplasmic domain (4). Within the ECD, mouse IL‑5 R alpha shares 71% and 86% aa sequence identity with human and rat IL‑5 R alpha , respectively. Alternate splicing of mouse IL‑5 R alpha generates soluble secreted forms which function as IL‑5 antagonists (4, 5). The high affinity receptor for IL‑5 is a complex that consists of the ligand binding IL‑5 R alpha and the transmembrane common  beta chain ( beta c/CD131) which is shared with the receptor complexes for IL‑3 and GM‑CSF (6). IL‑5 R alpha binds IL‑5 at low affinity and then associates with preformed beta c oligomers to form the signaling‑competent receptor complex (7). IL‑5 stimulation of CD34⁺ hematopoietic progenitor cells induces the up‑regulation of transmembrane IL‑5 R alpha followed by eosinophilic differentiation and activation (8‑10). IL‑5 R alpha also promotes the differentiation of basophils and B cells (11, 12). Exposure of mature eosinophils to IL‑5 attenuates their IL‑5 responsiveness by inducing the down‑regulation of surface IL‑5 R alpha and increased production of soluble IL‑5 R alpha (13, 14). Elevated production of IL‑5 at sites of allergic inflammation induces eosinophilia and exacerbation of immune cell infiltration, tissue damage, and remodeling (2, 3).

1. Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
2. Rothenberg, M.E. and S.P. Hogan (2005) Annu. Rev. Immunol. 24:147.
3. Elsas, X.P. and M.I.G. Elsas (2007) Curr. Med. Chem. 14:1925.
4. Takaki, S. et al. (1990) EMBO J. 9:4367.
5. Cameron, L. et al. (2000) J. Immunol. 164:1538.
6. Tavernier, J. et al. (1991) Cell 66:1175.
7. Zaks-Zilberman, M. et al. (2008) J. Biol. Chem. 283:13398.
8. Tavernier, J. et al. (2000) Blood 95:1600.
9. Clutterbuck, E.J. et al. (1989) Blood 73:1504.
10. Lopez, A.F. et al. (1988) J. Exp. Med. 167:219.
11. Denburg, J.A. et al. (1991) Blood 77:1462.
12. Hasbold, J. et al. (2004) Nat. Immunol. 5:55.
13. Gregory, B. et al. (2003) J. Immunol. 170:5359.
14. Liu, L.Y. et al. (2002) J. Immunol. 169:6459.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.