205-IL)." class="big_lightbox"> 205-IL)." alt="Human IL-5 R alpha /CD125 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-253-NA) neutralizes IL 5-induced proliferation in the TF-1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 0.0800-0.800 µg/mL in the presence of 0.5 ng/mL Recombinant Human IL-5 (205-IL)." class="big_thumb" />

F0108). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> F0108). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

F0102B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0102B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins." alt="HEK293 human embryonic kidney cell line transfected with either (A) human IL-5 Ra/CD125 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human IL-5 Ra/CD125 Monoclonal Antibody (Catalog # MAB253) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

F0101B). Quadrant markers were set based on control antibody staining (MAB002). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_lightbox"> F0101B). Quadrant markers were set based on control antibody staining (MAB002). Staining was performed using our Staining Membrane-associated Proteins protocol." alt="HEK293 human embryonic kidney cell line transfected with (A) human IL-5 R alpha/CD125 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human IL-5 R alpha/CD125 Monoclonal Antibody (Catalog # MAB2531) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB002). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_thumb" />

205-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-5 Ra/CD125 Monoclonal Antibody (MAB2531). The ND50 is typically 0.05-0.5 µg/mL." class="big_lightbox"> 205-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-5 Ra/CD125 Monoclonal Antibody (MAB2531). The ND50 is typically 0.05-0.5 µg/mL." class="big_thumb" />