IL-36Ra/IL-1F5 Antibody (759207) [Alexa Fluor® 532]

• Reactivity: Mu
• Applications: WB
• Clone: 759207
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 532

IL-36Ra/IL-1F5 Antibody (759207) [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant mouse IL-36Ra/IL-1F5
  Met1-Cys155
  Accession # Q9QYY1
• Specificity: Detects mouse IL-36Ra/IL-1F5 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human IL-36Ra and recombinant mouse IL-1ra is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-36Ra/IL-1F5 Antibody (759207) [Alexa Fluor® 532]

• FIL1 delta
• FIL1
• FIL1-delta, IL-1RP3, IL-1L1, IL-1-delta)
• FIL1DIL-1 delta
• IL1F5 (Canonical product IL-1F5a)
• IL1F5
• IL-1HY1
• IL-1L1
• IL-1RP3
• IL36Ra
• IL-36Ra
• IL36RN
• interleukin 1 family, member 5 (delta)
• interleukin 1, delta
• Interleukin 36 Receptor Antagonist
• interleukin-1 family member 5
• Interleukin-1 HY1
• Interleukin-1 receptor antagonist homolog 1
• Interleukin-1-like protein 1
• Interleukin-36 Receptor Antagonist

Background

Mouse interleukin-36 receptor antagonist (IL-36Ra; previously IL-1F5; also named FIL-1δ [delta], IL-1HY1, IL-1H3, and IL-1L1) is a member of the IL-1 family of proteins (1-6). IL-1 family members include IL-1 beta , IL-1 alpha , IL-1ra, IL-18 and IL-1F5 through F10 (6, 7). All family members show a 12 beta -strand, beta -trefoil configuration, and are believed to have arisen from a common ancestral gene that underwent multiple duplications (8). The mouse IL-36Ra/IL-1F5 gene maps to a region on mouse chromosome 2 that contains all other IL-1 family members (except IL-18), supporting an evolutionary relationship with the IL-1 family (1, 9). It is particularly close to the gene for IL-1ra and is likely a relatively recent duplication of that gene. IL-36Ra/IL-1F5 is synthesized as a 156 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site(s) (2, 5, 8). Nevertheless, it appears to be secreted as a 17 kDa monomer. In humans, there is an alternate start site that potentially gives rise to an alternate splice form (5). This translated product has a premature stop codon, resulting in a truncated 16 aa peptide. Mouse to human, full length IL-36Ra/IL-1F5 has 90% aa identity. Within the family, IL-36Ra/IL-1F5 is 48%, 30%, 35%, 35%, 35%, 37% and 43% aa identical to IL-1ra, IL-1 beta , IL-36 alpha /IL-1F6, IL-37/IL-1F7, IL-36 beta /IL-1F8, IL-36 gamma /IL-1F9 and IL-1F10, respectively. Cells reported to express IL-36Ra/IL-1F5 include monocytes, B cells, dendritic cells/Langerhans cells, keratinocytes, and gastric fundus Parietal and Chief cells (1, 8). Indirect evidence suggests the receptor for IL-36Ra/IL-1F5 is IL-1 Rrp2 and/or IL-1 RAcP (9). IL-36Ra/IL-1F5 was initially reported to be an antagonist of IL-36 gamma activity (4, 7). This would be consistent with its hypothesized relationship to IL-1ra.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9QYY1()