IL-36Ra/IL-1F5 Antibody (759207) [Alexa Fluor® 532]

• Reactivity: Mu
• Applications: WB
• Clone: 759207
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 532

IL-36Ra/IL-1F5 Antibody (759207) [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant mouse IL-36Ra/IL-1F5
  Met1-Cys155
  Accession # Q9QYY1
• Specificity: Detects mouse IL-36Ra/IL-1F5 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human IL-36Ra and recombinant mouse IL-1ra is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-36Ra/IL-1F5 Antibody (759207) [Alexa Fluor® 532]

• FIL1 delta
• FIL1
• FIL1-delta, IL-1RP3, IL-1L1, IL-1-delta)
• FIL1DIL-1 delta
• IL1F5 (Canonical product IL-1F5a)
• IL1F5
• IL-1HY1
• IL-1L1
• IL-1RP3
• IL36Ra
• IL-36Ra
• IL36RN
• interleukin 1 family, member 5 (delta)
• interleukin 1, delta
• Interleukin 36 Receptor Antagonist
• interleukin-1 family member 5
• Interleukin-1 HY1
• Interleukin-1 receptor antagonist homolog 1
• Interleukin-1-like protein 1
• Interleukin-36 Receptor Antagonist

Background

Mouse interleukin-36 receptor antagonist (IL-36Ra; previously IL-1F5; also named FIL-1δ [delta], IL‑1HY1, IL-1H3, and IL-1L1) is a member of the IL‑1 family of proteins (1-6). IL‑1 family members include IL-1 beta , IL-1 alpha , IL-1ra, IL‑18 and IL‑1F5 through F10 (6, 7). All family members show a 12 beta -strand, beta -trefoil configuration, and are believed to have arisen from a common ancestral gene that underwent multiple duplications (8). The mouse IL‑36Ra/IL‑1F5 gene maps to a region on mouse chromosome 2 that contains all other IL-1 family members (except IL‑18), supporting an evolutionary relationship with the IL-1 family (1, 9). It is particularly close to the gene for IL-1ra and is likely a relatively recent duplication of that gene. IL‑36Ra/IL‑1F5 is synthesized as a 156 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site(s) (2, 5, 8). Nevertheless, it appears to be secreted as a 17 kDa monomer. In humans, there is an alternate start site that potentially gives rise to an alternate splice form (5). This translated product has a premature stop codon, resulting in a truncated 16 aa peptide. Mouse to human, full length IL‑36Ra/IL‑1F5 has 90% aa identity. Within the family, IL-36Ra/IL-1F5 is 48%, 30%, 35%, 35%, 35%, 37% and 43% aa identical to IL‑1ra, IL‑1 beta , IL‑36 alpha /IL‑1F6, IL‑37/IL‑1F7, IL-36 beta /IL-1F8, IL‑36 gamma /IL‑1F9 and IL‑1F10, respectively. Cells reported to express IL-36Ra/IL-1F5 include monocytes, B cells, dendritic cells/Langerhans cells, keratinocytes, and gastric fundus Parietal and Chief cells (1, 8). Indirect evidence suggests the receptor for IL-36Ra/IL-1F5 is IL‑1 Rrp2 and/or IL-1 RAcP (9). IL-36Ra/IL-1F5 was initially reported to be an antagonist of  IL-36 gamma activity (4, 7). This would be consistent with its hypothesized relationship to IL‑1ra.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.