Recombinant Mouse IL-36 beta/IL-1F8 Protein

SDS-Page: Mouse IL-36 beta/IL-1F8 183 a.a Protein [NBP2-35130]

• Reactivity: Mu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Mouse IL-36 beta/IL-1F8 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 183 amino acids corresponding to IL-36 beta/IL-1F8 Source: E. coli

  Uniprot ID: Q9D6Z6

  Amino Acid Sequence: MMAFPPQSCV HVLPPKSIQM WEPNHNTMHG SSQSPRNYRV HDSQQMVWVL TGNTLTAVPA SNNVKPVILS LIACRDTEFQ DVKKGNLVFL GIKNRNLCFC CVEMEGKPTL QLKEVDIMNL YKERKAQKAF LFYHGIEGST SVFQSVLYPG WFIATSSIER QTIILTHQRG KLVNTNFYIE SEK

• Details of Functionality: IL-36 beta/IL-1F8 protein is fully biologically active when compared to standard. The specific activity determined by its ability in a functional ELISA. Immobilized rIL-36 beta/IL-1F8 at 1 ug/mL can bind recombinant murine IL-1 Rrp2 with a range of 0.15-5 ug/mL.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IL36B
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of IL-36 beta/IL-1F8 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 20.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris, 300 mM NaCl, pH 8.0.
• Preservative: 5% Trehalose
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Mouse IL-36 beta/IL-1F8 Protein

• family of interleukin 1-eta
• FIL1 eta
• FIL1
• FIL1-(ETA)
• FIL1H
• FILI-(ETA)
• IL-1 eta
• IL1-ETA
• IL1F8 (Canonical product IL-1F8a)
• IL-1F8 (FIL1-eta)
• IL1F8
• IL-1F8
• IL1H2
• IL-1H2
• IL36 beta
• IL-36 beta
• IL36B
• interleukin 1 family, member 8 (eta)
• interleukin 1, eta
• Interleukin 36, Beta
• Interleukin-1 eta
• interleukin-1 family member 8
• Interleukin-1 homolog 2
• Interleukin-1 Superfamily e
• Interleukin-36 Beta

Background

Interleukin-36 (IL-36) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36alpha, IL-36beta, and IL-36gamma (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. IL-36 beta is reported to be expressed at higher levels in psoriatic plaques than in symptomless psoriatic skin or healthy control skin and it can stimulate production of interleukin-6 and interleukin-8 in synovial fibrobasts, articular chondrocytes and mature adipocytes. It beta has two isoforms. IL-36beta isoform 2 contains one potential N-linked glycosylation site in its C-terminus, while IL -36beta isoform 1 lacks potential N-linked glycosylation sites and four of the conserved beta-strands. Within the IL-1 family, IL-36beta/IL-1F8 shares 30 %, 32 %, 37 %, 46 %, 34 %, 45 % and 28 % a.a. sequence identity with IL-1 ra, IL-1beta, IL-36Ra/IL-1F5, IL-36alpha/IL-1F6, IL-37/IL-1F7, IL-36gamma/IL-1F9 and IL-1F10, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IL36B
• Uniprot:
  • Q9D6Z6()
  • Q9D6Z6()
  • Q9D6Z6()