Recombinant Human IL-36 beta/IL-1F8 Protein

SDS-Page: Human IL-36 beta/IL-1F8 157 a.a Protein [NBP2-34887]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human IL-36 beta/IL-1F8 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 157 amino acids corresponding to IL-36 beta/IL-1F8 Source: E. coli

  Uniprot ID: Q9NZH7-2

  Amino Acid Sequence: MNPQREAAPK SYAIRDSRQM VWVLSGNSLI AAPLSRSIKP VTLHLIACRD TEFSDKEKGN MVYLGIKGKD LCLFCAEIQG KPTLQLKEKN IMDLYVEKKA QKPFLFFHNK EGSTSVFQSV SYPGWFIATS TTSGQPIFLT KERGITNNTN FYLDSVE

• Details of Functionality: IL-36 beta/IL-1F8 protein is fully biologically active when compared to standard. The specific activity is determined by its binding ability in a functional ELISA. Immobilized IL-36 beta/IL-1F8 at 1 ug/mL can bind recombinant human IL-1 Rrp2 Fc Chimera with a range of 0.15-5 ug/mL.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IL36B
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of IL-36 beta/IL-1F8 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 17.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human IL-36 beta/IL-1F8 Protein

• family of interleukin 1-eta
• FIL1 eta
• FIL1
• FIL1-(ETA)
• FIL1H
• FILI-(ETA)
• IL-1 eta
• IL1-ETA
• IL1F8 (Canonical product IL-1F8a)
• IL-1F8 (FIL1-eta)
• IL1F8
• IL-1F8
• IL1H2
• IL-1H2
• IL36 beta
• IL-36 beta
• IL36B
• interleukin 1 family, member 8 (eta)
• interleukin 1, eta
• Interleukin 36, Beta
• Interleukin-1 eta
• interleukin-1 family member 8
• Interleukin-1 homolog 2
• Interleukin-1 Superfamily e
• Interleukin-36 Beta

Background

Interleukin-1 beta (IL-1B) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1a and IL-1B binds to the same receptor and has similar but not identical biological properties; The mature human IL-1B shares 96% amino acid sequence identity with rhesus and 67 % -78 % with canine, mouse and rat IL-1B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for IL-36 beta/IL-1F8 Recombinant Protein (NBP2-34887). (Showing 1 - 1 of 1 FAQ).

1. Could you let us know the following information?1) Lyophilized buffer formulation (e.g. Lyophilized from PBS.)2) Preservative/Concentration
   • This protein is supplied in the lyophilized format from the same associated lab of ours, and can be used in the functional assays in vivo (so should not have any preservative and endotoxin-tested. Our lab's databases show that E. coli, lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, and no preservative.

Bioinformatics

• Gene Symbol: IL36B