Recombinant Human IL-36 beta/IL-1F8 Protein

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SDS-Page: Human IL-36 beta/IL-1F8 157 a.a Protein [NBP2-34887]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

Order Details

Recombinant Human IL-36 beta/IL-1F8 Protein Summary

Description
A single, non-glycosylated polypeptide chain corresponding to 157 residues of IL-36 beta/IL-1F8.

Source: Escherichia coli

Amino Acid Sequence: MNPQREAAPK SYAIRDSRQM VWVLSGNSLI AAPLSRSIKP VTLHLIACRD TEFSDKEKGN MVYLGIKGKD LCLFCAEIQG KPTLQLKEKN IMDLYVEKKA QKPFLFFHNK EGSTSVFQSV SYPGWFIATS TTSGQPIFLT KERGITNNTN FYLDSVE

Preparation
Method
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
Details of Functionality
IL36 Beta Protein is fully biologically active when compared to standard. The specific activity is determined by its binding ability in a functional ELISA. Immobilized rHuIL-36beta at 1 ug/mL can bind recombinant human IL-1 Rrp2 Fc Chimera with a range of 0.15-5 ug/mL.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
IL36B
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of rHuIL-36beta, 157a.a. as determined by LAL method.

Applications/Dilutions

Theoretical MW
17.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative
No Preservative
Concentration
LYOPH
Purity
> 97 % pure by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Notes

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Alternate Names for Recombinant Human IL-36 beta/IL-1F8 Protein

  • family of interleukin 1-eta
  • FIL1 eta
  • FIL1
  • FIL1-(ETA)
  • FIL1H
  • FILI-(ETA)
  • IL-1 eta
  • IL1-ETA
  • IL1F8 (Canonical product IL-1F8a)
  • IL-1F8 (FIL1-eta)
  • IL1F8
  • IL-1F8
  • IL1H2
  • IL-1H2
  • IL36 beta
  • IL-36 beta
  • IL36B
  • interleukin 1 family, member 8 (eta)
  • interleukin 1, eta
  • Interleukin 36, Beta
  • Interleukin-1 eta
  • interleukin-1 family member 8
  • Interleukin-1 homolog 2
  • Interleukin-1 Superfamily e
  • Interleukin-36 Beta

Background

Interleukin-1 beta (IL-1B) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1a and IL-1B binds to the same receptor and has similar but not identical biological properties; The mature human IL-1B shares 96% amino acid sequence identity with rhesus and 67 % -78 % with canine, mouse and rat IL-1B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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FAQs for IL-36 beta/IL-1F8 Protein (NBP2-34887). (Showing 1 - 1 of 1 FAQ).

  1. Could you let us know the following information?1) Lyophilized buffer formulation (e.g. Lyophilized from PBS.)2) Preservative/Concentration
    • This protein is supplied in the lyophilized format from the same associated lab of ours, and can be used in the functional assays in vivo (so should not have any preservative and endotoxin-tested. Our lab's databases show that E. coli, lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, and no preservative.

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Bioinformatics

Gene Symbol IL36B