Recombinant Human IL-36 beta/IL-1F8 Protein Summary
A single, non-glycosylated polypeptide chain corresponding to 157 residues of IL-36 beta/IL-1F8.
Source: Escherichia coli
Amino Acid Sequence: MNPQREAAPK SYAIRDSRQM VWVLSGNSLI AAPLSRSIKP VTLHLIACRD TEFSDKEKGN MVYLGIKGKD LCLFCAEIQG KPTLQLKEKN IMDLYVEKKA QKPFLFFHNK EGSTSVFQSV SYPGWFIATS TTSGQPIFLT KERGITNNTN FYLDSVE
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
|Details of Functionality
IL36 Beta Protein is fully biologically active when compared to standard. The specific activity is determined by its binding ability in a functional ELISA. Immobilized rHuIL-36beta at 1 ug/mL can bind recombinant human IL-1 Rrp2 Fc Chimera with a range of 0.15-5 ug/mL.
| Protein/Peptide Type
> 97 % pure by SDS-PAGE and HPLC
Less than 1 EU/ug of rHuIL-36beta, 157a.a. as determined by LAL method.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
> 97 % pure by SDS-PAGE and HPLC
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions
Alternate Names for Recombinant Human IL-36 beta/IL-1F8 Protein
- family of interleukin 1-eta
- FIL1 eta
- IL-1 eta
- IL1F8 (Canonical product IL-1F8a)
- IL-1F8 (FIL1-eta)
- IL36 beta
- IL-36 beta
- interleukin 1 family, member 8 (eta)
- interleukin 1, eta
- Interleukin 36, Beta
- Interleukin-1 eta
- interleukin-1 family member 8
- Interleukin-1 homolog 2
- Interleukin-1 Superfamily e
- Interleukin-36 Beta
Interleukin-1 beta (IL-1B) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1a and IL-1B binds to the same receptor and has similar but not identical biological properties; The mature human IL-1B shares 96% amino acid sequence identity with rhesus and 67 % -78 % with canine, mouse and rat IL-1B.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed
for 3 months from date of receipt.
Customers Who Viewed This Item Also Viewed...
Applications: WB, Block
Applications: WB, Flow, CyTOF-ready
Applications: Flow, CyTOF-ready, ICC
Applications: Func, PAGE, Bioactivity
Publications for IL-36 beta/IL-1F8 Protein (NBP2-34887) (0)
There are no publications for IL-36 beta/IL-1F8 Protein (NBP2-34887).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for IL-36 beta/IL-1F8 Protein (NBP2-34887) (0)
There are no reviews for IL-36 beta/IL-1F8 Protein (NBP2-34887).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
Other Available Formats
Additional IL-36 beta/IL-1F8 Products
Bioinformatics Tool for IL-36 beta/IL-1F8 Protein (NBP2-34887)
Discover related pathways, diseases and genes to IL-36 beta/IL-1F8 Protein (NBP2-34887). Need help?
Read the Bioinformatics Tool Guide
for instructions on using this tool.
Blogs on IL-36 beta/IL-1F8