IL-36 beta/IL-1F8 Antibody Pair

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Cell Culture Supernatant
• Standard Curve Range: 15.6 - 1000 pg/ml
• Sensitivity: 10 pg/ml

IL-36 beta/IL-1F8 Antibody Pair Summary

• Description: Sandwich ELISA for the quantitative determination of Human IL-36 beta/IL-1F8
• Standard Curve Range: 15.6 - 1000 pg/ml
• Sensitivity: 10 pg/ml
• Assay Type: Sandwich ELISA
• Sample Volume: 100 ul
• Gene: IL36B

Applications/Dilutions

• Dilutions:
  • Sandwich ELISA
• Application Notes: 1. Dilute the desired amount of Coating Antibody (COAT) (1 mg/ml) (rabbit polyclonal antibody) to 5 ug/ml in PBS without carrier protein and use it fresh.
  2. Dilute the desired amount of Detection Antibody (DET) (0.5 mg/ml) (mouse monoclonal antibody) to 0.5 ug/ml in ELISA Buffer and use it fresh.
  3. Reconstitute the Streptavidin-HRP (STREP) with 100 ul ELISA Buffer. After reconstitution, prepare aliquots and store the reconstituted STREP at -20C. Avoid freeze/thaw cycles. Dilute the reconstituted Streptavidin-HRP to the working concentration by adding 10 ul in 10 ml of ELISA Buffer (1:1000).
  4. Reconstitute the Standard Protein (STD) (recombinant human IL-36 beta/IL-1F8) with 100 ul ELISA Buffer to obtain a concentration of 1 ug/ml. After reconstitution, prepare aliquots and store the reconstituted standard at -20C. Avoid freeze/ thaw cycles. A standard curve using 2-fold serial dilutions in ELISA Buffer is recommended. Suggested standard points are 1 ng/ml, 0.5 ng/ml, 0.25 ng/ml, 0.125 ng/ml, 0.062 ng/ml, 0.031 ng/ml, 0.0156 ng /ml and 0 ng/ml.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Alternate Names for IL-36 beta/IL-1F8 Antibody Pair

• family of interleukin 1-eta
• FIL1 eta
• FIL1
• FIL1-(ETA)
• FIL1H
• FILI-(ETA)
• IL-1 eta
• IL1-ETA
• IL1F8 (Canonical product IL-1F8a)
• IL-1F8 (FIL1-eta)
• IL1F8
• IL-1F8
• IL1H2
• IL-1H2
• IL36 beta
• IL-36 beta
• IL36B
• interleukin 1 family, member 8 (eta)
• interleukin 1, eta
• Interleukin 36, Beta
• Interleukin-1 eta
• interleukin-1 family member 8
• Interleukin-1 homolog 2
• Interleukin-1 Superfamily e
• Interleukin-36 Beta

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: IL36B