Recombinant Human IL-36 alpha/IL-1F6 Protein

SDS-Page: Recombinant Human IL-36 alpha/IL-1F6 Protein [NBP2-34880]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human IL-36 alpha/IL-1F6 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 158 amino acids corresponding to IL-36 alpha/IL-1F6 Source: E. coli

  Uniprot ID: Q9UHA7

  Amino Acid Sequence: MEKALKIDTP QQGSIQDINH RVWVLQDQTL IAVPRKDRMS PVTIALISCR HVETLEKDRG NPIYLGLNGL NLCLMCAKVG DQPTLQLKEK DIMDLYNQPE PVKSFLFYHS QSGRNSTFES VAFPGWFIAV SSEGGCPLIL TQELGKANTT DFGLTMLF

• Details of Functionality: IL36 alpha Protein is fully biologically active when compared to standard. The specific activity determined by its ability in a functional ELISA. Immobilized rHuIL-36alpha at 1 ug/mL can bind recombinant human IL-1 Rrp2 Fc Chimera with a range of 0.15-5 ug/mL.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IL36A
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of IL-36 alpha/IL-1F6 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 17.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human IL-36 alpha/IL-1F6 Protein

• FIL1 epsilon
• FIL1
• FIL1(EPSILON)
• FIL1E
• IL-1 epsilon
• IL1(EPSILON)
• IL1E
• IL-1E
• IL-1F6 (FIL-1-epsilon)
• IL1F6
• IL-1F6
• IL36 alpha
• IL-36 alpha
• IL36A
• interleukin 1 family, member 6 (epsilon)
• interleukin 1, epsilon
• Interleukin 36, Alpha
• Interleukin-1 epsilon
• interleukin-1 family member 6
• Interleukin-36 Alpha

Background

Interleukin-36 (IL-36) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36alpha, IL-36beta, and IL-36gamma (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. Studies showed IL-36alpha is mainly found in skin and lymphoid tissues, but also in fetal brain, trachea, stomach and intestine. Notably, IL-36 alpha is the only novel IL-1 family member expressed on Tcells. Recombinant human interleukin-36 alpha (153 a.a.) contains 153 amino acids residues which is a single non-glycosylated polypeptide and it shares 57 - 68 % a.a. sequence identity with mouse, rabbit, equine and bovine IL-36alpha and 27 - 57 % a.a. sequence identity with other novel IL-1 family members.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for IL-36 alpha/IL-1F6 Recombinant Protein (NBP2-34880). (Showing 1 - 1 of 1 FAQ).

1. Could you let us know the following information?1) Lyophilized buffer formulation (e.g. Lyophilized from PBS.)2) Preservative/Concentration
   • This protein is supplied in the lyophilized format from the same associated lab of ours, and can be used in the functional assays in vivo (so should not have any preservative and endotoxin-tested. Our lab's databases show that recombinant protein was produce in Escherichia coli with a plasmid containing the gene of interest. It is lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4, and no preservative of any kind.

Bioinformatics

• Gene Symbol: IL36A
• Uniprot:
  • Q9UHA7()
  • Q9UHA7()
  • Q9UHA7()