Recombinant Mouse IL-36 alpha/IL-1F6 Protein

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SDS-Page: Mouse IL-36 alpha/IL-1F6 153 a.a Protein [NBP2-35127]

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Func, PAGE
Format
Carrier-Free
Concentration
LYOPH

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Recombinant Mouse IL-36 alpha/IL-1F6 Protein Summary

Description
A single, non-glycosylated biologically active polypeptide chain corresponding to 153 residues of IL36A.

Source: E. coli

Amino Acid Sequence: RAASPSLRHV QDLSSRVWIL QNNILTAVPR KEQTVPVTIT LLPCQYLDTL ETNRGDPTYM GVQRPMSCLF CTKDGEQPVL QLGEGNIMEM YNKKEPVKAS LFYHKKSGTT STFESAAFPG WFIAVCSKGS CPLILTQELG EIFITDFEMI VVH

Preparation
Method
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
Details of Functionality
IL36 alpha Protein is fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion in murine NIH/3T3 cells is less than 25 ng/ml, corresponding to a specific activity of > 4.0 x 10^4 IU/mg.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
IL36A
Purity
>95% pure by SDS-PAGE
Endotoxin Note
Less than 1 EU/ug of endotoxin as determined by LAL method.

Applications/Dilutions

Theoretical MW
17.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20C. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in PBS, 1 mM DTT, 3 % trehalose.
Preservative
Trehalose
Concentration
LYOPH
Purity
>95% pure by SDS-PAGE
Reconstitution Instructions
Reconstitute with sterilized distilled water or 0.1% BSA aqueous buffer to a final concentration of 0.1 - 1.0 mg/ml.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Recombinant Mouse IL-36 alpha/IL-1F6 Protein

  • FIL1 epsilon
  • FIL1
  • FIL1(EPSILON)
  • FIL1E
  • IL-1 epsilon
  • IL1(EPSILON)
  • IL1E
  • IL-1E
  • IL-1F6 (FIL-1-epsilon)
  • IL1F6
  • IL-1F6
  • IL36 alpha
  • IL-36 alpha
  • IL36A
  • interleukin 1 family, member 6 (epsilon)
  • interleukin 1, epsilon
  • Interleukin 36, Alpha
  • Interleukin-1 epsilon
  • interleukin-1 family member 6
  • Interleukin-36 Alpha

Background

Interleukin-36 (IL-36) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36alpha, IL-36beta, and IL-36gamma (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. IL-36alpha is mainly found in skin and lymphoid tissues, but also in fetal brain, trachea, stomach and intestine. It is expressed by monocytes, B and T cells. Notably, IL-36 alpha is the only novel IL-1 family member expressed on T-cells. Recombinant murine interleukin-36 alpha contains 153 amino acids residues which is a single nonglycosylated polypeptide. Specifically, mouse IL-36alpha shares 83 % a.a. sequence identity with rat IL-36alpha, 54 - 60 % with human, rabbit, equine and bovine IL-36alpha.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol IL36A
Entrez
Uniprot