IL-3 Antibody (4815R) [Unconjugated]

Recombinant Human IL-3 (Catalog # 203-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by the Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-3 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-3 Recombinant Monoclonal Antibody (Catalog # MAB603R). The ND50 is typically0.1-0.3 µg/mL.

• Reactivity: Hu
• Applications: B/N, ELISA(Cap)
• Clone: 4815R
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IL-3 Antibody (4815R) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: E. coli-derived recombinant human IL-3
  Ala20-Phe152
  Accession # P08700
• Specificity: Detects human IL-3 in ELISAs and Western blots. In sandwich ELISAs, no significant cross-reactivity or interference with recombinant human (rh) IL-1 alpha , rhIL-1 beta , recombinant mouse (rm) IL-1 beta , rhIL-2, rhIL-3 R alpha , rhIL-3 R beta , rmIL-3, rhIL-4, rmIL-4, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, bovine FGF acidic, bovine FGF basic, rhG-CSF, rhGM-CSF, rmGM-CSF, rhLIF, hPDGF, porcine PDGF, human TGF‑ beta 1, porcine
  TGF‑ beta 1, rhTGF‑ beta 1, porcine TGF‑ beta 2, porcine TGF‑ beta 1.2, rhTNF-alpha , or rhTNF-beta was observed. Does not neutralize the biological activity of
  rmIL‑3.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL3
• Purity Statement: Protein A or G purified from cell culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.1-0.3 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-3 Antibody (4815R) [Unconjugated]

• Hematopoietic growth factor
• IL3
• IL-3
• IL-3MGC79398
• interleukin 3 (colony-stimulating factor, multiple)
• interleukin-3
• Mast cell growth factor
• mast-cell growth factor
• MCGF
• MCGFMGC79399
• MULTI-CSF
• multilineage-colony-stimulating factor
• Multipotential colony-stimulating factor
• P-cell stimulating factor
• P-cell-stimulating factor

Background

Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor, P-cell stimulating factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factor and WEHI-3 growth factor. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes can also produce IL-3. At the amino acid sequence level, mature human and murine IL-3 share only 29% sequence identity. Consistent with this lack of homology, IL-3 activity is highly species-specific and human IL-3 does not show activity on murine cells.

IL-3 exerts its biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-3 signaling is composed of at least two subunits, an IL-3 specific alpha chain which binds IL-3 with low affinity and a common beta chain that is shared by the IL-5 and GM-CSF high-affinity receptors. Although the beta chain itself does not bind IL-3, it confers high-affinity IL-3 binding in the presence of the  alpha chain. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils and various myeloid leukemic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-3 Antibody (MAB603R)(1)

Publication using MAB603R

• Kankaanranta H, Ilmarinen P, Zhang X, Adcock I, Lahti A, Barnes P, Giembycz M, Lindsay M, Moilanen E Tumour necrosis factor-alpha regulates human eosinophil apoptosis via ligation of TNF-receptor 1 and balance between NF-kappaB and AP-1. PLoS ONE, 2014-02-28;9(2):e90298. 2014-02-28 [PMID: 24587316] (Neutralization, Human)

Bioinformatics

• Gene Symbol: IL3
• Uniprot:
  • P08700()