Recombinant Mouse IL-27 Protein

Product Discontinued

Recombinant Mouse IL-27 Protein Summary

• Description: Recombinant bioactive protein containing 207 amino acids for Mouse IL-27
  
  Source:E. coli
  Amino Acid Sequence:(Q8K3I6)
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab269237)
• Preparation Method: Determined to be >97% pure by SDS-PAGE
• Details of Functionality: Measured by dose-dependent inhibition of TGF-B & Il-6-induced IL-17A expression in mouse CD4 splenocytes. 50 ng/ml of mouse p28 is capable of inhibiting >25% of Il-17A expression in this assay.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IL27
• Purity: >97%, by SDS-PAGE
• Endotoxin Note: <0.01 ng/ug

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 23.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 10 mM sodium phosphate (pH 7.5)
• Concentration: Lyoph
• Purity: >97%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute in H2O to 0.1 - 0.5 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for long-term storage.

Alternate Names for Recombinant Mouse IL-27 Protein

• IL-27 p28 subunit
• IL-27 subunit alpha
• IL27
• IL-27
• IL27A
• IL-27-A
• IL27-A
• IL27p28
• IL30
• interleukin 27
• interleukin 30
• interleukin-27 subunit alpha
• MGC71873
• p28IL-27A

Background

Recombinant mouse IL-27/p28 Subunit, also known as Interleukin-30, is a member of the IL-12 family of Cytokines. When combined with EBI3 (Epstein-Barr virus induced gene 3), the heterodimer formed is IL-27. Mouse p28 is a proinflammatory cytokine inducing immunomodulatory effects. Current research is underway to delineate specific biological functions. Recombinant mouse p28 is a single-chain 207 amino acid peptide with a mass of approximately 23.7 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IL27