IL-24 Antibody (303308)

IL-24 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse IL-24 Monoclonal Antibody (Catalog # MAB2786) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Mu
• Applications: WB, ICC/IF
• Clone: 303308
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-24 Antibody (303308) Summary

• Immunogen: E. coli-derived recombinant mouse IL-24
  Arg27-His181
  Accession # NP_444325
• Specificity: Detects mouse IL-24 in direct ELISAs and Western blots. No cross-reactivity with recombinant human IL-24 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: IL24
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-24 Antibody (303308)

• C49A
• FISP
• IL10B
• IL24
• IL-24
• interleukin 24
• MDA-7
• MDA7MOB5
• melanocyte-associated Mda-7
• melanoma differentiation association protein 7
• Melanoma differentiation-associated gene 7 protein
• MOB-5
• ST16
• ST16interleukin-24
• suppression of tumorigenicity 16 (melanoma differentiation)
• Suppression of tumorigenicity 16 protein

Background

Mouse IL-24, also known as melanoma differentiation-associated gene 7 (MDA-7) and Th2-specific cytokine FISP, is a 27 kDa secreted glycoprotein that is a member of the IL-10 family. It is synthesized as a precursor with a large pro-region that is removed to generate a 155 aa mature protein. Mature mouse IL-24 shares 84% and 69% amino acid identity with rat and human IL-24, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-24 Antibody (MAB2786)(6)

Publications using MAB2786

• Wang, B;Xia, Y;Zhou, C;Zeng, Y;Son, HG;Demehri, S; CD4+ T helper 2 cell-macrophage crosstalk induces IL-24-mediated breast cancer suppression JCI insight 2025-01-09 [PMID: 39782693] (Immunofluorescence, Mouse)
• Christopher Sie, Ravi Kant, Christian Peter, Andreas Muschaweckh, Monika Pfaller, Lucy Nirschl et al. IL-24 intrinsically regulates Th17 cell pathogenicity in mice Journal of Experimental Medicine 8/1/2022 [PMID: 35819408]
• LZ Rao, Y Wang, L Zhang, G Wu, L Zhang, FX Wang, LM Chen, F Sun, S Jia, S Zhang, Q Yu, JH Wei, HR Lei, T Yuan, J Li, X Huang, B Cheng, J Zhao, Y Xu, BW Mo, CY Wang, H Zhang IL-24 deficiency protects mice against bleomycin-induced pulmonary fibrosis by repressing IL-4-induced M2 program in macrophages Cell Death Differ, 2020-11-03;0(0):. 2020-11-03 [PMID: 33144678] (IHC, Mouse)
• CY Chang, HJ Shih, IT Huang, PS Tsai, KY Chen, CJ Huang Magnesium Sulfate Mitigates the Progression of Monocrotaline Pulmonary Hypertension in Rats Int J Mol Sci, 2019-09-18;20(18):. 2019-09-18 [PMID: 31540416] (IHC-P, Rat)
• AR Burmeister, MB Johnson, JJ Yaemmongko, I Marriott Murine astrocytes produce IL-24 and are susceptible to the immunosuppressive effects of this cytokine J Neuroinflammation, 2019-03-02;16(1):55. 2019-03-02 [PMID: 30825881] (Western Blot, Mouse)
• Uto-Konomi A, Miyauchi K, Ozaki N Dysregulation of suppressor of cytokine signaling 3 in keratinocytes causes skin inflammation mediated by interleukin-20 receptor-related cytokines. PLoS ONE, 2012-07-05;7(7):e40343. 2012-07-05 [PMID: 22792286] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: IL24
• Uniprot:
  • NP_444325()