IL-22R alpha 1 Antibody - BSA Free

Western Blot: IL-22R alpha 1 Antibody [NBP1-76724] - Analysis of IL-22 receptor expression in human HepG2 cell lysate with IL-22 receptor antibody at (A) 0.5 and (B) 1 ug /ml.

Immunohistochemistry: IL-22R alpha 1 Antibody [NBP1-76724] - Staining of rat kidney tissue with antibody at 5 ug/mL.

Immunocytochemistry/Immunofluorescence: IL-22R alpha 1 Antibody [NBP1-76724] - Immunofluorescence:HepG2 cells with IL-22RA1 receptor antibody at 10 ug/ml.

Immunocytochemistry/ Immunofluorescence: IL-22R alpha 1 Antibody - BSA Free [NBP1-76724] - Immunofluorescence of IL-22R alpha 1 in rat kidney tissue with IL-22R alpha 1 antibody at 20 u/ml.

Immunocytochemistry/ Immunofluorescence: IL-22R alpha 1 Antibody - BSA Free [NBP1-76724] - Immunofluorescence of IL-22 receptor in HepG2 cells with IL-22 receptor antibody at 10 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

IL-22R alpha 1 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human IL-22 receptor precursor. The immunogen is located within the first 50 amino acids of IL-22 Receptor. Amino Acid Squence: QSSNFENILTWDSGPE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IL22RA1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Western Blot 0.5-1 ug/ml
• Application Notes: Use in Flow Cytometry reported in scientific literature (PMID:32127533).
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  IL-22R alpha 1 Protein (NBP1-76724PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (88%). Use in Mouse reported in scientific literature (PMID: 32127533). Use in Mouse reported in scientific literature (PMID:32127533).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for IL-22R alpha 1 Antibody - BSA Free

• CRF2-9
• CRF2-9interleukin 22 receptor
• Cytokine receptor class-II member 9
• Cytokine receptor family 2 member 9
• IL-22 R alpha 1
• IL-22 receptor subunit alpha-1
• IL22R alpha 1
• IL22R
• IL22R1
• IL22RA1
• IL-22Ra1
• IL-22R-alpha-1
• IL-TIF-R1
• interleukin 22 receptor, alpha 1
• interleukin-22 receptor subunit alpha-1
• zcytoR11

Background

A novel cytokine, designated IL-TIF for IL-10 related T cell-derived inducible factor and IL-22, was recently identified. The receptor for IL-22 (IL-22R, also termed CRF2-9 and IL-TIF-R1 chain) is a new member of the class II cytokine receptor family. IL-22R forms a complex with IL-10 receptor beta chain and mediates IL-22 signaling. IL-22 and its receptor activate JAK-STAT signaling pathway. IL22R is expressed in normal liver and kidney and their cell lines HepG2 and TK-10. A soluble form of IL-22 receptor, also termed IL-22 binding protein (IL-22BP) and IL-22 receptor-alpha 2 (IL-22RA2), was identified very recently. IL-22BP prevents binding of IL-22 to the functional cell surface IL-22R complex and neutralizes IL-22 activity. LPS induces IL-22 expression, which indicates the role of IL-22 in inflammatory response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-22R alpha 1 Antibody (NBP1-76724)(1)

Publication using NBP1-76724

• Yang J, Yang X, Wang L, Li M B cells control lupus autoimmunity by inhibiting Th17 and promoting Th22 cells Cell Death Dis 2020-03-03 [PMID: 32127533] (FLOW, Mouse)

Bioinformatics

• Gene Symbol: IL22RA1
• Uniprot:
  • AF286095(Human)