IL-20 Antibody (380605) [Unconjugated]

Recombinant Mouse IL‑20 (Catalog # 1204-ML) stimulates proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑20 R alpha and IL‑20 R beta in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑20 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑20 Monoclonal Antibody (Catalog # MAB12043). The ND50 is typically 2-8 µg/mL.

• Reactivity: Mu
• Applications: B/N
• Clone: 380605
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-20 Antibody (380605) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse IL‑20
  Leu25-Leu176
  Accession # Q9JKV9.1
• Specificity: Detects mouse IL-20 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: IL20
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-20 Antibody (380605) [Unconjugated]

• Cytokine Zcyto10
• IL10D
• IL20
• IL-20
• IL-20ZCYTO10four alpha helix cytokine
• interleukin 20
• interleukin-20
• MGC96907

Background

Mouse Interleukin 20 (IL-20) was identified by searching sequence databases for translated sequences with a signal sequence and amphipathic helices found in helical cytokines. Based on the human molecule, mouse IL-20 was discovered in a skin library. Mouse IL-20 is synthesized as a 176 amino acid (aa) precursor that contains a 24 aa signal sequence and a 152 aa mature segment. There are no N-linked glycosylation sites and it is doubtful that the native molecule is glycosylated. Although IL-20 is a distant member of the IL-10 family, it functions as a monomer. IL-20 shares less than 40% aa sequence identity with other IL-10 family members. Mouse and human IL-20 are 77% aa identical in the mature segment. IL-20 production has been found in skin and trachea. In particular, activated keratinocytes and, possibly, monocytes are reported to express IL-20. There are two heterodimeric receptor complexes for IL-20. The first complex is composed of IL-20 R alpha and IL-20 R beta . The second complex is composed of IL-22 R and IL-20 R beta . Whereas the IL-22 R/IL-20 R beta complex is shared with IL-24/mda-7, the IL-20 R alpha /IL-20 R beta complex is shared with both IL-19 and IL-24. Little is known about the function of IL-20. It is reported to induce the proliferation of multipotential hematopoietic progenitor cells, direct the differentiation and expansion of keratinocytes, and promote the release of pro-inflammatory mediators in keratinocytes and other IL-20 receptor expressing cells (1-6).

1. Blumberg, H. et al. (2001) Cell 104:9.
2. Liu, L. et al. (2003) Blood 102:3206.
3. Rich, B.E. and T.S. Kupper (2001) Curr. Biol. 11:R531.
4. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
5. Dumoutier, L. (2001) J. Immunol. 167:3545. 
6. Romer, J. (2003) J. Invest. Dermatol. 121:1306.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-20 Antibody (MAB12043)(2)

Publications using MAB12043

• JR Dayton, Y Yuan, LP Pacumio, BG Dorflinger, SC Yoo, MJ Olson, SI Hernández-, MM McMahon, L Cruz-Oreng Expression of IL-20 Receptor Subunit beta Is Linked to EAE Neuropathology and CNS Neuroinflammation Frontiers in Cellular Neuroscience, 2021-09-07;15(0):683687. 2021-09-07 [PMID: 34557075] (In Vivo, Mouse)
• Uto-Konomi A, Miyauchi K, Ozaki N Dysregulation of suppressor of cytokine signaling 3 in keratinocytes causes skin inflammation mediated by interleukin-20 receptor-related cytokines. PLoS ONE, 2012-07-05;7(7):e40343. 2012-07-05 [PMID: 22792286] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: IL20
• Uniprot:
  • Q9JKV9.1()