IL-1ra/IL-1F3/IL1RN Antibody [Biotin]

• Reactivity: Eq
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IL-1ra/IL-1F3/IL1RN Antibody [Biotin] Summary

• Immunogen: Recombinant Equine IL‑1ra/IL‑1F3
  His26-Gln177
  Accession # O18999.1
• Specificity: Detects equine IL-1ra in ELISAs and Western blots. In sandwich ELISAs, approximately 20% cross‑reactivity is observed with rmIL-1ra, less than 5% cross‑reactivity with rrIL-1ra, less than 2% cross‑reactivity with rpIL-1ra, and less than 0.4% cross‑reactivity with rhIL-1ra is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL1RN
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Equine IL-1ra/IL-1F3 Antibody (Catalog # AF2466)
  ELISA Detection: Equine IL-1ra/IL-1F3 Biotinylated Antibody (Catalog # BAF2466)
  Standard: Recombinant Equine IL-1ra/IL-1F3 (Catalog # 2466-RA)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1ra/IL-1F3/IL1RN Antibody [Biotin]

• DIRA
• ICIL-1ra
• IL1F3
• IL-1F3
• IL1ra
• IL-1ra
• IL-1ra3
• IL1RN
• IL-1RN
• interleukin 1 receptor antagonist
• IRAP
• MVCD4

Background

Secreted equine IL-1 receptor antagonist (IL-1ra) is a presumably 22 - 25 kDa glycoprotein produced by variety of cell types that antagonizes IL-1 activity (1 - 3). It is a member of the IL-1 family of proteins that includes IL-1 alpha and IL-1 beta . Although there is little amino acid (aa) identity (< 30%) among the three IL-1 family members, all molecules bind to the same receptors, all show a beta -trefoil structure, and all are believed to have evolved from a common ancestral gene (1 - 4). Equine IL-1ra is synthesized as a 177 aa precursor that contains a 25 aa signal sequence plus a 152 aa mature region. There is one intrachain disulfide bond and one potential N-linked glycosylation site (3, 5, 6). Mature equine sIL-1ra is 78%, 78%, 80%, 82%, and 76% aa identical to mature mouse, human, porcine, canine and bovine IL-1ra, respectively. In human, three non-secreted IL-1ra isoforms have also been identified. It is unknown if such an analogous situation exists in equine. Cells known to secrete IL-1ra include fibroblasts, vascular smooth muscle cells, intestinal columnar epithelium, chondrocytes, macrophages, mast cells, neutrophils and hepatocytes.

There are two type I transmembrane glycoprotein receptors for IL-1ra. The first is the bioactive 80 kDa type I IL-1 receptor (IL-1 RI), and the second is the inert (decoy) 65 kDa type II IL-1 receptor. IL-1ra binding to IL-1 RI competitively blocks IL-1 ( alpha or beta ) binding to the same receptor. This results in receptor ligation without activation (1, 7). The type II IL-1 receptor is inert, and any binding of IL-1ra not only fails to block co-existing IL-1 activity, but may actually potentiate it by removing an IL-1 antagonist. Functionally, all activities attributed to IL-1ra are explained by its role as a competitive inhibitor of IL-1 binding to IL-1 RI (1, 2, 8, 9).

1. Arend, W.P. et al. (1998) Annu. Rev. Immunol. 16:27.
2. Roux-Lombard, P. (1998) Eur. Cytokine Netw. 9:565.
3. Dayer-J-M. (2002) Clin. Exp. Rheumatol. 20(27):S14.
4. Eisenberg, S.P. et al. (1991) Proc. Natl. Acad. Sci. USA 88:5232.
5. Kato, H. et al. (1997) Vet. Immunol. Immunopathol. 56:221.
6. Howard, R.D. et al. (1998) Am. J. Vet. Res. 59:712.
7. Dinarello, C.A. (1997) Semin. Oncol. (Suppl 9):S9.
8. Irikura, V.M. et al. (2002) J. Immunol. 169:393.
9. Arend, W.P. and C. Gabay (2000) Arth. Res. 2:245.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL1RN
• Uniprot:
  • O18999.1()