IL-1ra/IL-1F3/IL1RN Antibody (694204)

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line treated with 1 µg/mL LPS for 24 hours. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse IL-1ra/IL-1F3 Monoclonal Antibody (Catalog # MAB4801) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-1ra/IL-1F3 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB
• Clone: 694204
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-1ra/IL-1F3/IL1RN Antibody (694204) Summary

• Immunogen: E. coli-derived recombinant mouse IL-1ra/IL-1F3
  Arg8-Gln159
  Accession # Q542W1
• Specificity: Detects mouse IL-1ra/IL-1F3 in direct ELISAs and Western blots. In Western blots, approximately 30%-50% cross-reactivity with recombinant equine IL-1ra and recombinant human (rh) IL-1ra is observed. Less than 5% cross-reactivity with recombinant mouse (rm) IL-1b, rmIL-18, rmIL-36Ra, rmIL-36a, rmIL-36b, rmIL-1F10, rhIL-36, rhIL-1F7, or recombinant porcine IL-1ra, or recombinant rat IL-1ra is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: IL1RN
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1ra/IL-1F3/IL1RN Antibody (694204)

• DIRA
• ICIL-1ra
• IL1F3
• IL-1F3
• IL1ra
• IL-1ra
• IL-1ra3
• IL1RN
• IL-1RN
• interleukin 1 receptor antagonist
• IRAP
• MVCD4

Background

IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally-occurring, fully glycosylated form has an apparent molecular weight of about 25 kDa. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha . It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells. In mouse, IL-1 RII does not bind IL-1ra. The recombinant, non-glycosylated form of IL-1ra blocks binding of IL-1 to its receptor equally as well as the naturally-occurring, glycosylated form. The IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Pre-clinical and clinical studies were done to test possible therapeutic applications for IL-1ra in the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1ra/IL-1F3/IL1RN Antibody (MAB4801)(3)

Publications using MAB4801

• Ge, X;Han, H;Desert, R;Das, S;Song, Z;Komakula, SSB;Chen, W;Athavale, D;Lantvit, D;Nieto, N; A PROTEIN COMPLEX OF LIVER ORIGIN ACTIVATES A PRO-INFLAMMATORY PROGRAM THAT DRIVES HEPATIC AND INTESTINAL INJURY IN ALCOHOL-ASSOCIATED LIVER DISEASE Cellular and molecular gastroenterology and hepatology 2024-05-22 [PMID: 38788899] (Functional Assay, Transgenic Mouse)
• K Tsujimaru, M Takanashi, K Sudo, A Ishikawa, S Mineo, S Ueda, K Kumagai, M Kuroda Extracellular microvesicles that originated adipose tissue derived mesenchymal stem cells have the potential ability to improve rheumatoid arthritis on mice Regenerative Therapy, 2020-12-09;15(0):305-311. 2020-12-09 [PMID: 33426233] (Western Blot, Mouse)
• M William, LP Leroux, V Chaparro, J Lorent, TE Graber, MN M'Boutchou, T Charpentie, A Fabié, CM Dozois, S Stäger, LC van Kempen, T Alain, O Larsson, M Jaramillo eIF4E-Binding Proteins 1 and 2 Limit Macrophage Anti-Inflammatory Responses through Translational Repression of IL-10 and Cyclooxygenase-2 J. Immunol., 2018-04-30;0(0):. 4/30/2018 [PMID: 29712774]

Bioinformatics

• Gene Symbol: IL1RN
• Uniprot:
  • Q542W1()