IL-1ra/IL-1F3/IL1RN Antibody (114801) [Alexa Fluor® 700]

• Reactivity: Po
• Applications: WB
• Clone: 114801
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

IL-1ra/IL-1F3/IL1RN Antibody (114801) [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant porcine IL‑1ra/IL‑1F3
  His26-Gln177
  Accession # Q29056
• Specificity: Detects porcine IL‑1ra/IL‑1F3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse IL-1ra/IL-1F3 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1ra/IL-1F3/IL1RN Antibody (114801) [Alexa Fluor® 700]

• DIRA
• ICIL-1ra
• IL1F3
• IL-1F3
• IL1ra
• IL-1ra
• IL-1ra3
• IL1RN
• IL-1RN
• interleukin 1 receptor antagonist
• IRAP
• MVCD4

Background

IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally-occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha . It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptors (I and II) on human cells, but reportedly will not block binding to the type II receptor on murine pre-B cell lines. IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Human, mouse and rat IL-1ra all share approximately 80% homology with porcine IL-1ra.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.