IL-1ra/IL-1F3/IL1RN Antibody (10309) [Unconjugated]


Recombinant Human IL‑1ra/IL‑1F3 (Catalog # 280-RA) inhibits Recombinant Human IL‑1 alpha /IL‑1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner more

Product Details

Reactivity HuSpecies Glossary
Applications B/N, ELISA(Cap)

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IL-1ra/IL-1F3/IL1RN Antibody (10309) [Unconjugated] Summary

E. coli-derived recombinant human IL‑1ra/IL-1F3
Accession # P18510
Detects human IL‑1ra/IL-1F3 in ELISAs. In ELISAs, this antibody shows less than 0.2% cross-reactivity with recombinant mouse (rm) IL‑1ra and no cross-reactivity with rhIL‑1 alpha, rpIL‑1 alpha, rmIL‑1 alpha, rrIL‑1 alpha, rhIL‑1 beta, rpIL‑1 beta, rmIL‑1 beta, rrIL‑1 beta, rhIL‑1 RI, or rhIL‑1 RII.
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Neutralization 10-20 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Human IL-1ra/IL-1F3 Antibody (Catalog # MAB280)
ELISA Detection: Human IL-1ra/IL-1F3 Biotinylated Antibody (Catalog # BAF280)
Standard: Recombinant Human IL-1ra/IL-1F3 (Catalog # 280-RA)
Read Publications using
MAB280 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1ra/IL-1F3/IL1RN Antibody (10309) [Unconjugated]

  • DIRA
  • ICIL-1ra
  • IL1F3
  • IL-1F3
  • IL1ra
  • IL-1ra
  • IL-1ra3
  • IL1RN
  • IL-1RN
  • interleukin 1 receptor antagonist
  • IRAP
  • MVCD4


IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells, but reportedly will not block binding to the type II receptor on murine pre-B cell lines. The recombinant, non-glycosylated form of IL-1ra blocks binding of IL-1 to its receptor equally as well as the naturally-occurring, glycosylated form. The IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Currently, pre-clinical and clinical studies are underway to test possible therapeutic applications for IL-1ra in the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-1ra/IL-1F3/IL1RN Antibody (MAB280)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: Array Development, ELISA Development, ICC, Neutralization.

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Showing Publications 1 - 6 of 6.
Publications using MAB280 Applications Species
JS Hook, M Cao, K Weng, N Kinnare, JG Moreland Mycobacterium tuberculosis Lipoarabinomannan Activates Human Neutrophils via a TLR2/1 Mechanism Distinct from Pam3CSK4 J. Immunol., 2019;0(0):. 2019 [PMID: 31871022] (Human) Human
Damien Dietrich Interleukin-36 potently stimulates human M2 macrophages, Langerhans cells and keratinocytes to produce pro-inflammatory cytokines Cytokine, 2016;84(0):88-98. 2016 [PMID: 27259168] (Neutralization, Human) Neutralization Human
Kalliolias GD, Gordon RA, Ivashkiv LB Suppression of TNF-alpha and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27. J. Immunol., 2010;185(11):7047-56. 2010 [PMID: 20971923] (ELISA Development, Human) ELISA Development Human
Palmer G, Trolliet S, Talabot-Ayer D, Mezin F, Magne D, Gabay C Pre-interleukin-1alpha expression reduces cell growth and increases interleukin-6 production in SaOS-2 osteosarcoma cells: Differential inhibitory effect of interleukin-1 receptor antagonist (icIL-1Ra1). Cytokine, 2005;31(2):153-60. 2005 [PMID: 15923123] (ICC, Human) ICC Human
Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC trans fatty acids and systemic inflammation in heart failure. Am. J. Clin. Nutr., 2004;80(6):1521-5. 2004 [PMID: 15585763] (ELISA Development, Human) ELISA Development Human
Copeland S, Siddiqui J, Remick D Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines. J. Immunol. Methods, 2004;284(1):99-106. 2004 [PMID: 14736420] (Array Development, Human) Array Development Human

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Gene Symbol IL1RN