IL-17RD/SEF Antibody

Western Blot: IL-17 RD/SEF Antibody [NBP2-16944] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with IL17 Receptor D antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: IL17RD Antibody [NBP2-16944] - Immunofluorescence analysis of methanol-fixed MCF-7, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: IL17RD Antibody [NBP2-16944] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.

Western Blot: IL17RD Antibody [NBP2-16944] - Sample (30 ug of whole cell lysate) A: IMR32 7. 5% SDS PAGE gel, diluted at 1:3000.

Product Discontinued

IL-17RD/SEF Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human IL17 Receptor D. The exact sequence is proprietary.
• Localization: Golgi apparatus membrane; Single-pass type I membrane protein; Cell membrane; Single-pass type I membrane protein; Isoform 4: Cytoplasm;
• Predicted Species: Mouse (92%), Bovine (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IL17RD
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 82 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for IL-17RD/SEF Antibody

• FLJ35755
• IL-17 RD
• IL17RD
• IL-17RD
• IL-17RDIL-17 receptor D
• IL17Rhom
• IL17RLM
• IL17RLMDKFZp434N1928
• interleukin 17 receptor D
• interleukin-17 receptor D
• Interleukin-17 receptor-like protein
• MGC133309
• Sef homolog
• SEF
• SEFhSef

Background

Fibroblast growth factors (FGFs; see MIM 603726) are secreted proteins involved in cellular proliferation, migration, differentiation, and survival. FGF activity is negatively regulated by members of the 'sprouty' family (e.g., SPRY1, MIM 602465). The SEF protein is a modulator of FGF signaling.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for IL-17RD/SEF Antibody (NBP2-16944)(1)

Publication using NBP2-16944

• Quadri JA, Sarwar S, Pinky et al. Fluoride induced tissue hypercalcemia, IL-17 mediated inflammation and apoptosis lead to cardiomyopathy: ultrastructural and biochemical findings Toxicology 2018-05-22 [PMID: 29800585] (IF/IHC, Rat)

Bioinformatics

• Gene Symbol: IL17RD
• Uniprot:
  • Q8NFM7()