IL-17RD/SEF Antibody (309539)

• Reactivity: Hu
• Applications: WB
• Clone: 309539
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-17RD/SEF Antibody (309539) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL‑17 RD/SEF
  Ala27-Arg299
  Accession # AAM77571
• Specificity: Detects human IL‑17 RD/SEF in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse IL-17 RD, recombinant human (rh) IL-17 RC, or rhIL-17B R is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL17RD
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17RD/SEF Antibody (309539)

• FLJ35755
• IL-17 RD
• IL17RD
• IL-17RD
• IL-17RDIL-17 receptor D
• IL17Rhom
• IL17RLM
• IL17RLMDKFZp434N1928
• interleukin 17 receptor D
• interleukin-17 receptor D
• Interleukin-17 receptor-like protein
• MGC133309
• Sef homolog
• SEF
• SEFhSef

Background

Interleukin-17 receptor D (IL-17 RD), also known as SEF (similar expression to FGFs), is a type I transmembrane protein that is found in both the cytoplasm and plasma membrane (1-5). The gene for this protein belongs to a synexpression group originally identified in zebrafish where SEF is expressed along with FGF-3, -8, sprouty-2 (SPRY2) and SPRY4 (6, 7). By alternate splicing, two transcript variants, potentially encoding three protein isoforms, exist. One is a full-length long form, one a shortened form that uses an alternate start site, and one an alternate splice form that removes the classic signal sequence (1-4). These isoforms have different expression patterns, subcellular localization, and function. The membrane-bound long form of human IL-17RD is synthesized as a 739 amino acid (aa) precursor protein with a putative 27 aa signal peptide, a 272 aa extracellular domain, a 20 aa transmembrane segment and a 420 aa cytoplastic domain. The extracellular domain contains one Ig-like domain and a fibronectin type III motif. The cytoplasmic domain shares homology with the intracellular domains of IL-17 receptor family members and shows one TIR (Toll/IL-1 Receptor) domain and a putative TRAF6-binding motif (2). Natural IL-17 RD has been shown to form homomultimeric complexes (3). Unlike the alternate splice form of IL-17 RD that has a restricted pattern of expression, the full-length IL-17 RD isoform is expressed in most adult tissues and during embryonic development (3, 5). Functionally, IL-17 RD has been shown to be an inhibitor of FGF signaling. The molecule’s extracellular domain does not seem to be involved. There is an interaction between the intracellular domains of FGFR1/2 and IL-17 RD that blocks ERK dissociation from MEK, thereby interfering with downstream ERK activation of nuclear Elk-1 (8). IL-17 RD has also been reported to interact with TAK1 and induce JNK activation and apoptosis (9). Ligands that interact with the extracellular domain of IL-17 RD have not been identified.

1. Furthauer, M. et al. (2002) Nat. Cell Biol. 4:170.
2. Xiong, S. et al. (2003) J. Biol. Chem. 278:50273.
3. Yang, R-B. et al. (2003) J. Biol. Chem. 278:33232.
4. Preger, E. et al. (2003) Proc. Natl. Acad. Sci. USA 101:1229.
5. Lin, W. et al. (2002) Mech. Dev. 113:163.
6. Tsang, M. et al. (2002) Nat. Cell Biol. 4:165.
7. Kovalenko, D. et al. (2003) J. Biol. Chem. 278:14087.
8. Torii, S. et al. (2004) Dev. Cell 7:33.
9. Yang, X. et al. (2004) J. Biol. Chem. 279:38099.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-17RD/SEF Antibody (MAB2275)(1)

Publication using MAB2275

• Darby S, Murphy T, Thomas H, Robson CN, Leung HY, Mathers ME, Gnanapragasam VJ Similar expression to FGF (Sef) inhibits fibroblast growth factor-induced tumourigenic behaviour in prostate cancer cells and is downregulated in aggressive clinical disease. Br. J. Cancer, 2009-11-03;101(11):1891-9. 2009-11-03 [PMID: 19888221] (IHC-P, Human)

Bioinformatics

• Gene Symbol: IL17RD
• Uniprot:
  • AAM77571()