IL-13R alpha 2 Antibody (2725C) [Unconjugated]

Western blot shows lysates of human prostate and human placenta. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human IL‑13 R alpha 2 Monoclonal Antibody (Catalog # MAB6142) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for IL‑13 R alpha 2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western lane view shows lysates of human placenta, loaded at 0.2 mg/mL. A specific band was detected for IL‑13 R alpha 2 at approximately 59 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human IL‑13 R alpha 2 Monoclonal Antibody (Catalog # MAB6142) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clone: 2725C
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

IL-13R alpha 2 Antibody (2725C) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Chinese Hamster Ovary cell line CHO-derived Human IL‑13 R alpha 2
  Met1-Leu342
  Accession # NP_000631
• Specificity: Detects Human IL‑13 R alpha 2 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 0.5 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-13R alpha 2 Antibody (2725C) [Unconjugated]

• cancer/testis antigen 19
• CD213a2 antigen
• CD213a2
• CT19
• IL-13 R alpha 2
• IL-13 receptor subunit alpha-2
• IL13BP
• IL13R alpha 2
• IL-13R subunit alpha-2
• IL13R
• IL-13R
• IL13RA2
• IL-13Ra2
• IL-13R-alpha-2
• interleukin 13 binding protein
• interleukin 13 receptor alpha 2 chain
• interleukin 13 receptor, alpha 2
• interleukin-13 receptor subunit alpha-2
• Interleukin-13-binding protein

Background

Interleukin‑13 Receptor alpha 2 (IL‑13 R alpha 2), also known as IL‑13 binding protein, and CD213a2, is a widely expressed 55 kDa cytokine receptor that plays an important role in the Th2‑polarized immune responses characteristic of a variety of pathologies, including parasitic infections and allergic asthma (1, 2). Mature human IL‑13 R alpha 2 consists of a 317 amino acid (aa) extracellular domain with three fibronectin type‑III domains, a WSxWS motif, a 20 aa transmembrane segment, and a 17 aa cytoplasmic domain (3). Within the ECD, human IL‑13 R alpha 2 shares 64% and 62% aa sequence identity with mouse and rat IL‑13 R alpha 2, respectively. In both mouse and human, a 40 kDa‑50 kDa soluble form of IL‑13 R alpha 2 can be generated by MMP‑8 mediated shedding in vitro (4).  Although this is assumed to occur in vivo in mouse, there is no evidence that shedding occurs in human (5‑7).  In mouse, alternative splicing also leads to sIL‑13 R alpha 2, but again, this phenomenon apparently does not occur in human (6‑7).  Thus, the biological effects of human IL‑13 R alpha 2 would appear to be mediated exclusively by membrane IL‑13 R alpha 2 (7). The biological effects of IL‑13 and IL‑4 are closely related in part due to a shared receptor system. IL‑13 binds to IL‑13 R alpha 1 which then forms a signaling complex with IL‑4 R alpha (8, 9). IL‑13 R alpha 2 functions as a decoy receptor by binding and internalizing IL‑13 and preventing it from signaling through the IL‑13 R alpha 1/IL‑4 R alpha complex (3, 10). IL‑13 R alpha 2 can also block IL‑4 induced responses by inhibiting IL‑4 bound IL‑13 R alpha 1/IL‑4 R alpha receptor complexes even though it does not itself bind IL‑4 (11, 12). Aside from its decoy function, IL‑13‑activated IL‑13 R alpha 2 directly promotes the development of tissue fibrosis by inducing the transcription of TGF‑ beta (13). Presumably, any human soluble IL‑13 R alpha 2, if it exists, will retain its ligand binding capability and attenuate responses to IL‑13 but not to IL‑4 (11, 14). The up‑regulation of transmembrane during Th2‑biased immune responses limits the extent of those responses (15‑17).

1. Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425.
2. Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338.
3. Caput, D. et al. (1996) J. Biol. Chem. 271:16921.
4. Chen, W. et al. (2008) J. Allergy Clin. Immunol. 122:625.
5. O’Toole, M. et al. (2008) Clin. Exp. Allergy 38:594.
6. Chen, W. et al. (2009) J. Immunol. 183:7870.
7. Kasaian, M.T. et al. (2011) J. Immunol. 187:561.
8. Andrews, A.-L. et al. (2006) J. Immunol. 176:7456.
9. Zurawski, S.M. et al. (1995) J. Biol. Chem. 270:13869.
10. Donaldson, D.D. et al. (1998) J. Immunol. 161:2317.
11. Andrews, A.-L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
12. Rahaman, S.O. et al. (2002) Cancer Res. 62:1103.
13. Fichtner-Feigl, S. et al. (2006) Nat. Med. 12:99.
14. Zhang, J.G. et al. (1997) J. Biol. Chem. 272:9474.
15. Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687.
16. Morimoto, M. et al. (2009) J. Immunol. 183:1934.
17. Zheng, T. et al. (2008) J. Immunol. 180:522.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • NP_000631()