Human IKK gamma ELISA Kit (Colorimetric)

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Standard Curve Range: 1.56 - 100 ng/mL (example only; lot dependent)
• Sensitivity: 0.67 ng/mL (example only; lot dependent)

Human IKK gamma ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 4.5 hours
• Standard Curve Range: 1.56 - 100 ng/mL (example only; lot dependent)
• Sensitivity: 0.67 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IKBKG

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human IKK gamma ELISA Kit (Colorimetric)

• FIP-3IP2
• FIP3NF-kappa-B essential modifier
• FIP3P
• I-kappa-B kinase subunit gamma
• IkB kinase gamma subunit
• IkB kinase subunit gamma
• IkB kinase-associated protein 1
• IKBKG
• IKK gamma
• IKKAP1
• IKKG
• IKK-gammaIPD2
• incontinentia pigmenti
• inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
• Inhibitor of nuclear factor kappa-B kinase subunit gamma
• IP
• IP1
• NEMO
• NEMOAMCBX1
• NFkappaB essential modulator
• NF-kappa-B essential modulator

Background

Familial incontinentia pigmenti (IP) is a genodermatosis that segregates as an X-linked dominant disorder and is usually lethal prenatally in males (The International Incontinentia Pigmenti Consortium, 2000 [PubMed 10839543]). In affected females it causes highly variable abnormalities of the skin, hair, nails, teeth, eyes, and central nervous system. The prominent skin signs occur in 4 classic cutaneous stages: perinatal inflammatory vesicles, verrucous patches, a distinctive pattern of hyperpigmentation, and dermal scarring. Cells expressing the mutated X chromosome are eliminated selectively around the time of birth, so females with IP exhibit extremely skewed X-inactivation. Familial incontinentia pigmenti is caused by mutations in the NEMO gene and is here referred to as IP2, or 'classical' incontinentia pigmenti. Sporadic incontinentia pigmenti, the so-called IP1, which maps to Xp11, is categorized as hypomelanosis of Ito (MIM 300337).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for IKK gamma ELISA Kit (NBP3-52626). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IKBKG