IKK gamma Antibody (4G9)

Images

 
Western Blot: IKK gamma Antibody (4G9) [H00008517-M01] - IKBKG monoclonal antibody (M01), clone 4G9 Analysis of IKBKG expression in K-562.
Immunocytochemistry/ Immunofluorescence: IKK gamma Antibody (4G9) [H00008517-M01] - Analysis of monoclonal antibody to IKBKG on HeLa cell. Antibody concentration 8 ug/ml.
Immunohistochemistry-Paraffin: IKK gamma Antibody (4G9) [H00008517-M01] - Analysis of monoclonal antibody to IKBKG on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.
Proximity Ligation Assay: IKK gamma Antibody (4G9) [H00008517-M01] - Analysis of protein-protein interactions between RALBP1 and IKBKG. HeLa cells were stained with anti-RALBP1 rabbit purified polyclonal 1:1200 and ...read more
Sandwich ELISA: IKK gamma Antibody (4G9) [H00008517-M01] - Detection limit for recombinant GST tagged IKBKG is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC-P, PLA
Clone
4G9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

IKK gamma Antibody (4G9) Summary

Immunogen
IKBKG (AAH50612, 1 a.a. - 419 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNRHLWKSQLCEMVQPSGGPAADQDVLGEESPLGKPAMLHLPSEQGAPETLQRCLEENQELRDAIRQSNQILRERCEELLHFQASQREEKEFLMCKFQEARKLVERLGLEKLDLKRQKEQALREVEHLKRCQQQMAEDKASVKAQVTSLLGELQESQSRLEAATKECQALEGRARAASEQARQLESEREALQQQHSVQVDQLRMQGQSVEAALRMERQAASEEKRKLAQLQVAYHQLFQEYDNHIKSSVVGSERKRGMQLEDLKQQLQQAEEALVAKQEVIDKLKEEAEQHKIVMETVPVLKAQADIYKADFQAERQAREKLAEKKELLQEQLEQLQREYSKLKASCQESARIEDMRKRHVEVSQAPLPPAPAYLSSPLALPSQRRSPPEEPPDFCCPKCQYQAPDMDTLQIHVMECIE
Specificity
IKBKG - inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
IKBKG
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Paraffin
  • Proximity Ligation Assay
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.
Antagonist

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for IKK gamma Antibody (4G9)

  • FIP-3IP2
  • FIP3NF-kappa-B essential modifier
  • FIP3P
  • I-kappa-B kinase subunit gamma
  • IkB kinase gamma subunit
  • IkB kinase subunit gamma
  • IkB kinase-associated protein 1
  • IKBKG
  • IKK gamma
  • IKKAP1
  • IKKG
  • IKK-gammaIPD2
  • incontinentia pigmenti
  • inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
  • Inhibitor of nuclear factor kappa-B kinase subunit gamma
  • IP
  • IP1
  • NEMO
  • NEMOAMCBX1
  • NFkappaB essential modulator
  • NF-kappa-B essential modulator

Background

Familial incontinentia pigmenti (IP) is a genodermatosis that segregates as an X-linked dominant disorder and is usually lethal prenatally in males (The International Incontinentia Pigmenti Consortium, 2000 [PubMed 10839543]). In affected females it causes highly variable abnormalities of the skin, hair, nails, teeth, eyes, and central nervous system. The prominent skin signs occur in 4 classic cutaneous stages: perinatal inflammatory vesicles, verrucous patches, a distinctive pattern of hyperpigmentation, and dermal scarring. Cells expressing the mutated X chromosome are eliminated selectively around the time of birth, so females with IP exhibit extremely skewed X-inactivation. Familial incontinentia pigmenti is caused by mutations in the NEMO gene and is here referred to as IP2, or 'classical' incontinentia pigmenti. Sporadic incontinentia pigmenti, the so-called IP1, which maps to Xp11, is categorized as hypomelanosis of Ito (MIM 300337).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Blogs on IKK gamma.

Regulating Immune Response Pathways with IKK beta
IKK beta, also known as IKK2, activates the NFkB complex by phosphorylating the NFkB inhibitor, IkBa. Several transcript variants, some protein-coding and some not, have been found for IKKB. The Nuclear Factor-kappa B (NF-kB) family of transcription f...  Read full blog post.

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Bioinformatics

Gene Symbol IKBKG
Entrez
OMIM
Uniprot