IKK beta Antibody

Product Discontinued

IKK beta Antibody Summary

• Immunogen: This antibody was made against a protein fragment from the C Terminus Region
• Specificity: This product is specific for Human IKBKB.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IKBKB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:5000-1:20000
• Application Notes: This antibody is useful in ELISA and Western Blot.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs. These products are available in limited quantities. Please enquire about the availability of this product before purchasing.

Alternate Names for IKK beta Antibody

• EC 2.7.11
• EC 2.7.11.10
• I-kappa-B kinase 2
• I-kappa-B-kinase beta
• IkBKB
• IKK beta
• IKK2
• IKK2FLJ38368
• IKK-B
• IKK-betaFLJ36218
• IKKBFLJ40509
• inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
• inhibitor of nuclear factor kappa-B kinase subunit beta
• MGC131801
• NFKBIKB
• NFKBIKBFLJ33771
• Nuclear factor NF-kappa-B inhibitor kinase beta

Background

IKK-beta (I-Kappa-B kinase-beta) is a member of the IKK complex which is composed of IKK-alpha, IKK-beta, IKK-gamma and IKAP (1-2). Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. IKK-beta has been shown to activate NF-kB and phosphorylate IKB-alpha and beta (3). Phosphorylation of 2 sites at the activation loop of IKK-beta is essential for activation of IKK by TNF and IL1. Once activated, IKK-beta autophosphorylates which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response (4). Additionally, IKK-beta activity can also be regulated by MEKK-1 (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IKK beta Antibody (21420002)(2)

Publications using 21420002

• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.

Bioinformatics

• Gene Symbol: IKBKB
• Uniprot:
  • AF080158(Human)