IKB zeta Antibody (OTI1E8) - Azide and BSA Free

Product Discontinued

IKB zeta Antibody (OTI1E8) - Azide and BSA Free Summary

• Immunogen: Full length recombinant protein of human IKB zeta (NP_113607) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NFKBIZ
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Western Blot 1:500
• Theoretical MW: 77.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for IKB zeta Antibody (OTI1E8) - Azide and BSA Free

• FLJ30225
• FLJ34463
• ikappaBzeta
• I-kappa-B-zeta
• IkappaB-zeta
• ikbzeta
• IkB-zeta
• IL-1 inducible nuclear ankyrin-repeat protein
• INAPIkappa B-zeta variant 3
• MAILIKBZikB-zeta
• Molecule possessing ankyrin repeats induced by lipopolysaccharide
• NF-kappa-B inhibitor zeta
• nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor

Background

IkappaBzeta (also known as MAIL and INAP) is an ankyrin-repeat-containing nuclear protein that is highly homologous to the IkappaB family member Bcl-3. Transcription of IkappaBzeta is upregulated by stimulation with TLR ligands, IL-1 and IL-6 in cultured B-lymphocytes and monocytes/macrophages, but only faintly so in T-lymphocytes, fibroblasts, and endothelial cells. IkappaB-zeta preferentially associates with the NF-kappaB subunit p50 rather than p65 and recombinant IkappaB-zeta proteins inhibit the DNA binding of the p65/p50 heterodimer and the p50/p50 homodimer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NFKBIZ