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IKB zeta Antibody (OTI1E8)

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Western Blot: IKB zeta Antibody (1E8) [NBP2-00642] HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IKB zeta (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IKB zeta.
Western Blot: IKB zeta Antibody (1E8) [NBP2-00642] HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IKB zeta (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Flow Cytometry: IKB zeta Antibody (1E8) [NBP2-00642] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IKB zeta antibody, and then ...read more
Flow Cytometry: IKB zeta Antibody (1E8) [NBP2-00642] - Analysis of Hela cells, using anti-IKB zeta antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: IKB zeta Antibody (1E8) [NBP2-00642] - Analysis of Jurkat cells, using anti-IKB zeta antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow
Clone
OTI1E8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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IKB zeta Antibody (OTI1E8) Summary

Immunogen
Full length human recombinant protein of human NFKBIZ (NP_113607) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NFKBIZ
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Flow Cytometry 1:100

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for IKB zeta Antibody (OTI1E8)

  • FLJ30225
  • FLJ34463
  • ikappaBzeta
  • I-kappa-B-zeta
  • IkappaB-zeta
  • ikbzeta
  • IkB-zeta
  • IL-1 inducible nuclear ankyrin-repeat protein
  • INAPIkappa B-zeta variant 3
  • MAILIKBZikB-zeta
  • Molecule possessing ankyrin repeats induced by lipopolysaccharide
  • NF-kappa-B inhibitor zeta
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor

Background

This gene is a member of the ankyrin-repeat family and is induced by lipopolysaccharide (LPS). The C-terminal portion of the encoded product which contains the ankyrin repeats, shares high sequence similarity with the I kappa B family of proteins. The latter are known to play a role in inflammatory responses to LPS by their interaction with NF-B proteins through ankyrin-repeat domains. Studies in mouse indicate that this gene product is one of the nuclear I kappa B proteins and an activator of IL-6 production. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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210-TA
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1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
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Simple Western lane view shows lysates of mouse lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Lipocalin‑2/NGAL at approximately 37 kDa (as indicated) using 5 µg/mL of Goat Anti-MouseLipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1857) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Western Blot: Bcl3 Antibody [NBP2-29946] - Western blot analysis of BCL3 Antibody (Center) (NBP2-29946) in mouse liver tissue lysates (35ug/lane). BCL3 (arrow) was detected using the purified Pab.
Immunocytochemistry/Immunofluorescence: Bcl3 Antibody [NBP2-29946] - Confocal immunofluorescent analysis of BCL3 Antibody (Center) (NBP2-29946) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Immunohistochemistry-Paraffin: Bcl3 Antibody [NBP2-29946] - Formalin-fixed and paraffin-embedded human lung carcinoma reacted with BCL3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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201-LB
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Human, Mouse, Rat
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NBP2-29661
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Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse spleen using CD4 antibody at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a strong signal in the membranes of a subset of cells in the spleen section and the staining showed punctate appearance in some cells.
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Western Blot: CEBP Beta Antibody (47A1) [NBP1-04266] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1 hour at room temperature. The membrane was probed with anti-CEBP beta 1:1000 in non-fat milk. This image was submitted via customer review.
Western Blot: CEBP Beta Antibody (47A1) [NBP1-04266] - NIH 3T3 cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CEBP Beta; antibody (1:1,000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
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Bioinformatics

Gene Symbol NFKBIZ
Entrez