IkB-epsilon Antibody (JM62-63)

Western Blot: IkB-epsilon Antibody (JM62-63) [NBP2-66903] - Analysis of IKB epsilon on different cell lysates using anti-IKB epsilon antibody at 1/1,000 dilution. Positive control: Line 1: THP-1 Line 2: Jurkat Line 3: Hela

Immunohistochemistry-Paraffin: IkB-epsilon Antibody (JM62-63) [NBP2-66903] - Analysis of paraffin-embedded human tonsil tissue using anti-IKB epsilon antibody. Counter stained with hematoxylin.

Product Discontinued

IkB-epsilon Antibody (JM62-63) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human IkB-epsilon aa 175-224 / 500. (SwissProt: O00221 Human)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NFKBIE
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for IkB-epsilon Antibody (JM62-63)

• epsilon
• ikappaBepsilon
• I-kappa-B-epsilon
• IkB-E
• IKBENF-kappa-B inhibitor epsilon
• IkBepsilon
• IkB-epsilon
• NF-kappa-BIE
• NFKBIE
• nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor

Background

The transcription factor NFkappaB is retained in the cytoplasm in an inactive form by the inhibitory protein IkappaB. Activation of NFkappaB requires that IkappaB be phosphorylated on specific serine residues, which results in targeted degradation of IkappaB. IkappaB kinase alpha (IKKalpha), previously designated CHUK, interacts with IkappaB-alpha and specifically phosphorylates IkappaB-alpha on the sites that trigger its degradation, Serines 32 and 36. IKKalpha appears to be critical for NFkappaB activation in response to proinflammatory cytokines. Phosphorylation of IkappaB by IKKalpha is stimulated by the NFkappaB inducing kinase (NIK), which itself is a central regulator for NFkappaB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKalpha, IKKbeta and IKKgamma (also designated NEMO), and each appear to make essential contributions to IkappaB phosphorylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NFKBIE