IkB-beta Antibody

Western Blot: IkB-beta Antibody [H00004793-D01P] - Analysis of NFKBIB expression in transfected 293T cell line by NFKBIB polyclonal antibody.Lane 1: NFKBIB transfected lysate(37.80 KDa).Lane 2: Non-transfected lysate.

Proximity Ligation Assay: IkB-beta Antibody [H00004793-D01P] - Analysis of protein-protein interactions between NFKBIB and FBXW11. HeLa cells were stained with anti-NFKBIB rabbit purified polyclonal 1:1200 and anti-FBXW11 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Product Discontinued

IkB-beta Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: NFKBIB (NP_002494.2, 1 a.a. - 356 a.a.) full-length human protein. MAGVACLGKAADADEWCDSGLGSLGPDAAAPGGPGLGAELGPGLSWAPLVFGYVTEDGDTALHLAVIHQHEPFLDFLLGFSAGTEYMDLQNDLGQTALHLAAILGETSTVEKLYAAGAGLCVAERRGHTALHLACRVGAHACARALLQPRPRRPREAPDTYLAQGPDRTPDTNHTPVALYPDSDLEKEEEESEEDWKLQLEAENYEGHTPLHVAVIHKDVEMVRLLRDAGADLDKPEPTCGRSPLHLAVEAQAADVLELLLRAGANPAARMYGGRTPLGSAMLRPNPILARLLRAHGAPEPEGEDEKSGPCSSSSDSDSGDEGDEYDDIVVHSSRSQTRLPPTPASKPLPDDPRPV
• Specificity: NFKBIB - nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NFKBIB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Proximity Ligation Assay
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for IkB-beta Antibody

• beta
• IkappaBbeta
• I-kappa-B-beta
• ikB-B
• IkBbeta
• IkB-beta
• IKBBikB-beta
• NF-kappa-B inhibitor beta
• NF-kappa-BIB
• NFKBIB
• nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor
• Thyroid receptor-interacting protein 9
• TR-interacting protein 9
• TRIP-9
• TRIP9ikappaBbeta

Background

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664 or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NFKBIB
• Entrez:
  • 4793(Human)
• Uniprot:
  • NP_002494.2(Human)