IkB-beta Antibody

Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, BaF3 mouse pro-B cell line, M1 mouse myeloid leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse IkB-beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5225) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IkB-beta at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Product Discontinued

IkB-beta Antibody Summary

• Immunogen: E. coli-derived recombinant mouse I kappa B-beta
  Met1-Ala359
  Accession # Q60778
• Specificity: Detects endogenous human and mouse I kappa B-beta in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NFKBIB
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IkB-beta Antibody

• beta
• IkappaBbeta
• I-kappa-B-beta
• ikB-B
• IkBbeta
• IkB-beta
• IKBBikB-beta
• NF-kappa-B inhibitor beta
• NF-kappa-BIB
• NFKBIB
• nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor
• Thyroid receptor-interacting protein 9
• TR-interacting protein 9
• TRIP-9
• TRIP9ikappaBbeta

Background

I kappa B-beta (I kappa B-beta; also NF-kappa-BIB) is a 46 kDa member of the I kappa B-beta inhibitor family of proteins. It is highly expressed in testicular spermatids, and serves to block NF-kappa B activity. This is accomplished by I kappa B-beta binding to either an NF-kappa B dimer, or a kappa B-Ras:NF-kappa B dimer complex. Mouse I kappa B-beta is 359 amino acids (aa) in length it contains a signal response region (aa 1‑50), six ankyrin repeats (aa 54‑308) and a PEST domain whose phosphorylation at Ser312 and Ser314 blocks NF‑ kappa B activation. Phosphorylation at Ser19 and Ser23 promotes I kappa B‑ beta ubiquitination and degradation. Full‑length mouse I kappa B‑ beta shares 94% and 84% aa identity with rat and human I kappa B-beta , respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IkB-beta Antibody (AF5225)(4)

Publications using AF5225

• Jeryl Sandoval, David J. Orlicky, Ayed Allawzi, Brittany Butler, Cynthia Ju, Caroline T. Phan et al. Toxic Acetaminophen Exposure Induces Distal Lung ER Stress, Proinflammatory Signaling, and Emphysematous Changes in the Adult Murine Lung Oxidative Medicine and Cellular Longevity 2019-11-28 [PMID: 31885815]
• Brittany Butler, Robyn De Dios, Leanna Nguyen, Sarah McKenna, Sankar Ghosh, Clyde J. Wright Developmentally Regulated Innate Immune NF kappa B Signaling Mediates IL-1 alpha Expression in the Perinatal Murine Lung Frontiers in Immunology 2019-07-10 [PMID: 31354715]
• Leanna Nguyen, Odalis Castro, Robyn De Dios, Jeryl Sandoval, Sarah McKenna, Clyde J. Wright Sex-differences in LPS-induced neonatal lung injury Scientific Reports 2019-06-11 [PMID: 31186497]
• Leanna Nguyen, Odalis Castro, Robyn De Dios, Jeryl Sandoval, Sarah McKenna, Clyde J. Wright Sex-differences in LPS-induced neonatal lung injury Scientific Reports 2019-06-11 [PMID: 31186497] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: NFKBIB
• Uniprot:
  • Q60778()