IkB-beta Antibody (7B4)

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Western Blot: IkB-beta Antibody (7B4) [NBP1-51637] - Western blot analysis using NFKBIB mouse mAb against Jurkat (1) cell lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
7B4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

IkB-beta Antibody (7B4) Summary

Immunogen
Purified recombinant fragment of human NFKBIB expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NFKBIB
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for IkB-beta Antibody (7B4)

  • beta
  • IkappaBbeta
  • I-kappa-B-beta
  • ikB-B
  • IkBbeta
  • IkB-beta
  • IKBBikB-beta
  • NF-kappa-B inhibitor beta
  • NF-kappa-BIB
  • NFKBIB
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor
  • Thyroid receptor-interacting protein 9
  • TR-interacting protein 9
  • TRIP-9
  • TRIP9ikappaBbeta

Background

Three major forms of IkB-like molecules have been identified and each is characterized by multiple copies of ankyrin repeats. IkB-alpha and IkB-beta appear to be the major regulatory forms of IkB in most cells. These proteins interact with p65 or c-Rel containing forms of NFkB and block nuclear import by masking the nuclear localization sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IkB. Immunoblotting easily detects the hyper-phosphorylated forms of IkB-alpha, but not phosphorylated IkB-beta.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NFKBIB
Entrez